We narrowed to 988 results for: Ran

1. DAP arrays for multiplex gene editing

   Type

   Blog Post

   ..., lengthy genetic payloads and insufficient transcription on the array have limited the scalability and...

2. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

   Type

   Blog Post

   ...these experiments because developing embryos are transparent, so it’s easy to watch development in real time...

3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   ...consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima ...

4. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   ...butyric acid (GABA) - the primary inhibitory neurotransmitter - is a powerful method for understanding how...

5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   Blog Post

   ...ADAs can inactivate the drug and increase drug clearance, lowering its efficacy and increasing the risk...

6. Four Factors that Differentiate the Stem Cell Field

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   Blog Post

   ...that stem cell scientists are so focused on translational research is encouraging. Patients are key to...

7. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   ... includes a 1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the 3.0kb fragment...

8. Finding and Joining Your Dream Lab

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   Blog Post

   ...where the topic of your research can make your transition easier – choose a lab that works on problems ...

9. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...experiments and to find tools that will make your transition into the field much easier! If there’s a plasmid...

10. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...spilling over" into another channel's detection range. Though you can compensate for this when reading...

11. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    ...P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies...

12. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ...increase your competitive edge, they alone, are no guarantee of success. Don’t luck into an internship, prepare...

13. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...in-house software and also eliminates many of the extraneous ORFs that cluttered our old plasmid maps. Clearly...

14. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post

    ... to research potential future employers and to arrange informational interviews. 4. “Tips for Scientists...

15. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    ...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows...

16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ...across a number of different sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto...

17. Clear and Accessible Writing

    Type

    Blog Post

    ...resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts...

18. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    ...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...

19. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    ...full of typos. Don’ts: Submit your manuscript to random journals or as many journals as possible at the...

20. Virus Protocol - Generating Stable Cell Lines

    Type

    Protocol

    ...Different brands and FBS lots can promote or inhibit transfection. Test a variety of brands and FBS lot...-term protein expression observed using transient transfection approaches, generating cell lines using...experiments in a stable cell line, as opposed to transiently-transfected cells, increases reproducibility, as it... the variation associated with repeated transient transfection. Some lentiviral vectors deliver mammalian...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...Pro-Tip Transducing too many cells relative to the number of virus particles reduces the transduction efficiency... may have 1 copy of the transgene, while others have >1 copy of the transgene in their genome. This can...