We narrowed to 1,082 results for: TOR;
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TypeBlog Post...You never know when you will meet a potential collaborator Some of these people you meet will review your...
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Transferable Skills Guide: Problem Solving
TypeBlog Post... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https... -
Antibodies 101: Fab Fragments
TypeBlog Post...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ... -
To Each HIS Own
TypeBlog Post...affinity purification of human insulin-like growth factor I from culture medium of E.coli. Bio/Technology... -
Plasmids 101: Cre-lox
TypeBlog Post...independently of any other accessory proteins or co-factors, thus allowing for broad applications in a variety... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...the appropriate primers to detect your targeting vector or gene of interest. 2. Breed your parental mice... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog Post...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog of the... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...quickly. Just make sure to incorporate the dilution factor in your calculation from OD to protein concentration... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...applied antibodies and need to dry the membrane for storage, but plan to reprobe it later, strip it thoroughly... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...impacts in the way we study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...sites. Often, the size of the plasmid insert and vector backbone are known and thus this technique can ...Restriction Digest If you clone an insert into a vector by a single restriction enzyme, you will need to...which orientation the insert is ligated into the vector backbone and therefore the only thing left to do... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...) For CrispyCrunch, we wanted to consider both factors, as a bench scientist would looking at a genome... -
Plasmids 101: Degron Tags
TypeBlog Post...usual. In the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could be less desirable... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...cut the vector, you will need to use a phosphatase to prevent re-circularization of the vector if you ...having difficulty finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut ... -
Immunocytochemistry
TypeProtocol...water Microscope slide Anti-fade mounting medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes...paraformaldehyde and follow your institution's laboratory safety guidelines for disposing of waste in the...remove the coverslip. Blot the coverslip with a laboratory wipe to remove excess liquid. Add 1 drop of anti-fade... -
Using a Light Microscope Protocol
TypeProtocol...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of... up your work area and microscope after use and store the microscope appropriately....