We narrowed to 1,050 results for: ins
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TypeBlog Post...Novartis Oncology. Bhang did her PhD work at Johns Hopkins and came to Novartis for her post-doc in January...
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Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that few... -
Rabies and Neuronal Tracing
TypeBlog Post...since these processes are mediated by other viral proteins), but the newly released RABV cannot infect any... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...certain cell types. Botond Roska’s lab at the Institute of Molecular and Clinical Ophthalmology Basel ... -
Engaging with science and society at pgEd
TypeBlog Post...organizations, libraries, youth groups, and faith institutions. The conversations often move quickly into sensitive... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...The day has arrived; you’ve painstakingly cared for your packaging cell line, prepped your DNA, transfected... -
Academic vs. Industry Postdocs
TypeBlog Post... decide what they’d like to work on later. For instance, AstraZeneca posts most of its positions all at... -
Tips for Writing a Good Cover Letter
TypeBlog Post...Highlight your achievements with specific examples. Instead of saying “I have excellent communication skills... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...genome-wide CRISPR screen presented in Morgens et al. contains three types of guides: 1) targeting guides (blue... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post...excellent posts on science career topics as well. Instead of rewriting or coming up with answers, I was able... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...two options: Film outside at a distance, or film inside with one person in a room. I wouldn’t be in there... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...and sarcasm, persistent teasing, name calling, insults, intimidation, sexual harassment Isolation – including... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...analysis plan when you design your experiment, instead of after you’ve already run it.) You don’t need... -
Neurodegeneration Plasmid Collection
TypeCollection...GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's L. Miguel...Miguel Martins 14124 pcDNA3-delta133HtrA-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14126 pET20b-delta133HtrA2...Miguel Martins 15939 pcDNA3-HtrA2-FLAG S142D HTRA2 Flag CMV Parkinson's L. Miguel Martins 15940 pcDNA3-HtrA2...Flag CMV Parkinson's L. Miguel Martins 16153 pET20b-delta133HtrA2 S142A HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16154 pET20b-delta133HtrA2 S142D HTRA2 His T7 Parkinson's L. Miguel Martins 16155 pET20b-...His T7 Parkinson's L. Miguel Martins 16156 pET20b-delta133HtrA2 S400D HTRA2 His T7 Parkinson's L. Miguel...Miguel Martins 16157 pET20b-delta133HtrA2 A141S HTRA2 His T7 Parkinson's L. Miguel Martins 16158 pET20b-... -
CRISPR Guide
TypeCollection...inactive “dead” Cas9 (dCas9) contains mutations in both nuclease domains, D10A and H840A, resulting in...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ...bidirectional insertions — meaning they may not be suitable for applications where orientation of the insert is ...exhibit almost unidirectional insertions but are more prone to off-target insertions. And in the case of both... edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor DNA at “...used to install the LSR Bxb1’s landing site into the genome at a desired locus, then Bxb1 installs the donor...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9... -
Antibody Guide
TypeCollection...four protein chains: two large heavy chains and two small light chains. These four domains come together...their monovalent nature. Darpins - Darpins are genetically engineered small proteins derived from ankyrin ...ankyrin repeat proteins. These are antibody mimics instead of modified antibodies and are not closely related...synthetic binding proteins which use the Fibronectin type III domain as their scaffolding instead of the antibody...Denature proteins, using heat and/or chemicals, and run on an SDS-PAGE gel to separate proteins by size...your antibody’s specificity. A blank (contains water or buffer instead of a biological sample), which captures... lab to specifically and tightly bind to other proteins. Antibodies, produced as part of the immune system... -
Bacterial Expression Systems
TypeCollection...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...recombinant proteins to the periplasmic space between the inner and outer membranes. Targeting proteins to the...Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living or fixed...Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent ...In BiFC, proteins of interest are fused to each part of a split FP. When the candidate proteins interact...Luciferase Fluorescent Biosensors Chromoproteins: Colorful Proteins For Molecular Biology Experiments ...Consortium page contains a variety of reagents for engineering and expressing zinc finger proteins. Addgene ... -
Luciferase Plasmid Collection
TypeCollection...Firefly Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5'...Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0 Firefly Insertion of target... Renilla Insertion of target sequence in the 3' UTR David Bartel 12177 pIS2 Renilla Insertion of target...construct that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return ...Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin fusion proteins for bioluminescent chemo-optogenetic...Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate... -
Immunology Research Plasmids and Resources
TypeCollection... E MGC4638 INS insulin ILPR, IRDN INS-IGF2 INS-IGF2 readthrough transcript - INSL3 insulin-like 3 (Leydig...MGC119819, RLF, RLNL INSL4 insulin-like 4 (placenta) EPIL, PLACENTIN INSL5 insulin-like 5 MGC126695, MGC126697...MGC126697, PRO182, UNQ156 INSL6 insulin-like 6 RIF1 INSR insulin receptor CD220, HHF5 JAG1 jagged 1 (Alagille...communication between cells of the immune system. Interleukins Interleukins are cytokines that are secreted by immune... omega 1 - IGF1 insulin-like growth factor 1 (somatomedin C) IGF1A, IGFI IGF1R insulin-like growth factor...MGC18216 IGF2 insulin-like growth factor 2 (somatomedin A) C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974 IGF2R...bA12D24.1.1, bA12D24.1.2 RLN3 relaxin 3 H3, RXN3, ZINS4, insl7 RNASE2 ribonuclease, RNase A family, 2 (liver... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...mCherry tagged fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector with ...designed for a user to insert a gene of interest. They may contain tags or fusion proteins, a multiple cloning...and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization...backbones curated by species, epitope tags/fusion proteins, function, selectable markers, and more. Plasmid...molecular biology to isolate, multiply, or express the insert they carry in the target cell. These vectors allow...function section for if you want to stably express an insert using viral vectors, are studying a different genetic...mammalian expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian...