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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: mpl

Showing: 881 - 900 of 938 results

  4. Lab to Office Culture Shock

    Type
    Blog Post
    ...friend: “I'm in the process of onboarding a new employee who's coming directly from grad school. The transition...

  7. March for Science

    Type
    Blog Post
    ...and public opinion do not seem to take note. For example, 91% of active research scientists (scientists ...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...empty NTC C AAV reference Sample 3 D E Sample 1 Sample 4 F E Sample 2 Sample 5 F Perform data analysis...most samples. If sample is expected to have a titer <1 x 10 12 GC/mL, use dilutions 3–6 If sample is expected...between them. Sample Data Figure 1: Example of a valid 8-point standard curve. Figure 2: Example of the amplification...standards and samples in duplicate at least If possible, include an AAV reference sample of known titer...the expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master...Count the number of samples (n) and prepare master mix for an additional 10 samples (n+10 – the additional...that there is enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use...

  13. Lentivirus ddPCR Titration

    Type
    Protocol
    ...concentration of each sample on a spectrophotometer. Prepare 25 ng/µL stocks of each sample. Samples can be used...constant across samples. In the RRE example below, 2-fold serial dilutions of a sample were loaded in ...tube as shown below. For 8 samples prepare enough master mix for 9 samples. Component Volume 9X Volume...layout is complete, select 'Run' to begin the droplet reading. When the droplet reading is complete, export...ddPCR Lentivirus sample data, RPP30 positive (green) and negative (black) droplets Sample ID Plasmid ID ... higher dilutions depending on their particular sample. This protocol was modified from the publication...Starting Thaw the master mix, primers/probe mixes and samples on ice before use. Wipe down all pipettes and surfaces...

  14. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...of 4X sample buffer to each sample. Add 2 µL 10X reducing agent to each sample. Spin the sample briefly...concentration of your samples based on your sample’s HC + LC area. Example calculation: If the sample HC + LC Area...250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated by denaturing...Invitrogen NP0001 4X NuPage sample buffer, Invitrogen NP0007 10X NuPage sample reducing agent (500 mM dithiothreitol...microcentrifuge. Heat the samples for 10 min at 100 °C in a heat block. Spin the sample briefly in the microcentrifuge...one lane before loading samples. Load 20 µL of each recombinant antibody sample to the appropriate well... mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed...

  15. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...of DNA (the template), a researcher can create thousands of identical copies using a simple set of reagents...If you are using the same template DNA for all your reactions, the Template DNA can be added to the master...particularly for GC-rich templates. What does each ingredient specifically do? Template DNA: Contains the portion...interested in amplifying for analysis and manipulation. It is essential that the template DNA and primer...Hybridize and are complementary to the 3’ ends of the sense and anti-sense strands of the template DNA. They ...polymerases from other species. In addition to the template DNA and the Taq polymerase, PCR requires free ... and downstream (3’) of the DNA segment to be amplified. When these reagents are combined in an appropriate...

  16. Kit Free RNA Extraction

    Type
    Protocol
    ... 10 seconds. Incubate sample(s) for 15 minutes on ice and centrifuge the sample(s) for 15 minutes at 12,000...Always wear gloves, and whenever possible, keep RNA sample and reagents cold and work quickly to reduce RNA...use 1 mL of Solution D per 1 X 10 7 cells. Allow sample(s) to sit at room temperature for 5 minutes to ...to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation will ...how effective your cell lysis protocol is. While simple homogenization is effective for most mammalian ... tissues such as bone, or bacteria/yeast/plant samples will require additional steps to effectively lyse...open the cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4 mL tube. ...

  17. Using a Light Microscope Protocol

    Type
    Protocol
    ...helps concentrate the light onto the sample, and then through the sample. However, some of that light won’...hitting the sample or b) using dyes or stains that add color to some components of the sample but not others... power objective to find your sample. Place your slide (or other sample type) on the microscope stage....so that the light shines onto your sample. To ensure that your sample is adequately illuminated, look through... coarse focus knob to bring your sample into focus. Once your sample is in focus, use the stage arm to... you can easily see your sample. Then use the focus knobs to bring the sample into focus. Pro-Tip As you...magnification used, any stains used in your sample, and sample information so that you or others can interpret...

  18. Centrifugation

    Type
    Protocol
    ... which you need to spin your samples. For example, spinning down samples in 1.5 mL microfuge tubes at ...liquid sample. It achieves this by using centrifugal force, which is generated by spinning the sample at ... with water to the same volume as your samples. If your samples are significantly higher density than ...centrifuge to separate different components in a liquid sample. Protocols...RNA extractions. Plus, centrifuges are useful for simply collecting liquid to the bottom of a tube. You ... containers, spin at different speeds, or keep samples at specific temperatures. This protocol will cover...for the biosafety level of the lab space Reagents Sample to be centrifuged Water Background Information ...

  19. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...finite binding capacity. If your sample exceeds the capacity, divide the sample among multiple columns. For...4 mL of recombinant antibody sample. Pro-Tip If the volume of the sample is greater than 4 mL , then divide... Slowly apply the sample to the center of the compact resin bed. Pro-Tip For sample volumes <1.5 mL top...the sample with 0.2 mL PBS to aid protein recovery. Allow the resin bed to fully absorb the sample. Centrifuge...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still... to see if the sample has reached the desired concentration. Gently transfer the sample from the reservoir... 250 mL sterile bottle, VWR 430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520...

  20. AAV ddPCR Titration

    Type
    Protocol
    ... ddPCR sample data Sample Dilution Concentration Titer (GC/mL) Average Titer (GC/mL) AAV Sample 1 6400000...tube as shown below. For 8 samples prepare enough master mix for 9 samples. ITR Master Mix Volume 9X Volume...layout is complete, select 'Run' to begin the droplet reading. When the droplet reading is complete, export...prepared for each AAV sample, and the three final dilutions are assayed. The samples that are assayed are...across the dilutions. In the example below, 2-fold serial dilutions of a sample were loaded in wells A04,... higher dilutions depending on their particular sample. For additional tips on AAV titering using ddPCR... on the insert. Please ensure that you are in compliance with your institution’s biosafety regulations...
Showing: 881 - 900 of 938 results