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We narrowed to 982 results for: ran

Showing: 881 - 900 of 982 results
  1. Fluorescence Titering Assay

    Type
    Protocol
    ...Different brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots... were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells...cells transduced F = Fraction of cells with fluorescence D = Dilution Factor V T = Transduction Volume...Workflow Timeline Day 0: Seed 293T cells Day 1: Transduce cells Day 2 (am): Remove media, replace with fresh...to the media, as it can affect viability and transduction efficiency. Titer will vary between cell lines...1468.5 1.5 1:75 20 1478.5 1.5 1:100 15 1483.5 1.5 Untransduced 0 1498.5 1.5 Mix the dilutions well Gently aspirate... one dilution with one well remaining as the untransduced control). Count the cells in the remaining well...
  2. Clear and Accessible Writing

    Type
    Blog Post
    ...resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts...
Showing: 881 - 900 of 982 results