We narrowed to 1,046 results for: sta

1. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...randomly, or at fixed genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

3. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...constructs are expressed transiently by transfection or stably impacts the success of BiFC experiments. In either...negative controls because their expression level, stability, and subcellular localization may differ from ...

4. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...

5. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   Blog Post

   ...role as a receptor for PHP.B. The Deverman lab started by seeing how changes in LY6A’s expression affected... References Holmes, Christina, and William L. Stanford. "Concise review: stem cell antigen‐1: expression...

6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ... T’s. Thus, it’s important that there are no “bystander bases” if you choose this system.  Fig....demonstrated that targeting dCas9 to transcriptional start sites was sufficient to repress transcription by...

7. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   ...Tong, Wang, et al., 2023). Based on this promising start, the team used rational mutagenesis to produce the...narrowed the editing window and reduced A → G bystander edits. Their final tool, ACBE-Q (for an N108Q ...

8. Hot Plasmids - August 2020

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   Blog Post

   ...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward...

9. Using Video to Share Your Science: We Share Science

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   Blog Post

   ...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...

10. CRISPR Activation: A Practical Guide

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    ...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to...

11. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ...yourself up for a screening success? Before you start analyzing individual clones, it’s wise to quickly...can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual...

12. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...split vectors has been inconsistent. A greater understanding of basic AAV biology, however, would help improve...and effective in the retina of a mouse model of Stargardt disease. Hum Mol Genet 24:6811–6825 . https://...

13. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ... to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving...evolution of the coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry ...

14. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...NPPOC-caged trimethoprim (TMP) Cas9 fused to destabilizing domains (Cas9-DHFR) Gene editing, transcription...

15. Chemogenetics Guide

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    Guide

    ...they also retain the ability to be activated by standard optogenetics workflows (such as laser or LED illumination...

16. New Coomassie Protocol Video

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    Blog Post

    ...out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol...

17. New Antibodies in the Lab Animation!

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    Blog Post

    ...applications in your experiment...but not sure where to start or what your options are? Addgene's latest animation...

18. Plasmids 101 eBook 4th Edition

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    Blog Post

    ...they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ...

19. Addgene Launches Partnership with IPI

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    Blog Post

    ...antibody protocols, and Antibodies 101 blog series.  Stay tuned for more information on our blog and social...

20. Antibodies 101 Series on TikTok

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    Blog Post

    ...our videos will also be shared on our @addgene Instagram account, or you can watch the full animation on...