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...Insert Vector Type Promoter PI Return to top Additional Resources Optogenetics Guide Viral Vector Packaging...optogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter Eligible for Viral Packaging PI Return...

...in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories will ensure that...post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the...service that we, as scientists, provide for journal editors to help decide whether work is suitable for publication... submission to the subsequent journal.  As a postdoctoral researcher studying plant development and abiotic...the data can be deposited in the public on-line repository (like GitHub, Zenodo, Figshare, Triad) while ... and Records: The Royal Society Journal of the History of Science 69.3 (2015): 337-352. PubMed PMID: 26495581...protocols Find eBooks on plasmids, CRISPR, viral vectors, and more ...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate... set on top of filing cabinets, a rickety old incubator, a few pipettes, and some home-made equipment....Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone... became our bacterial transformation guru and instructor for our intro to biohacking courses. Our members...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

...therefore we are not a party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate ...and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty years...with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795 MTAs...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...your organization agrees to let Addgene receive, store, and distribute your material under the specified...move forward with shipping the material to the requestor.  Why the MTA?  The MTA process can be a bit confusing...

...licensed distributor) allow you to quickly transfer a DNA sequence from the transposon vector to one of...encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful resource....such as with signaling molecules or transcription factors, or when the gene is constitutively-active or a...edit of interest. With the larger piggyBac™ donor vector, it is possible to make the ITR and selection cassette...re-cutting. Transposase removal of the cassette later restores the target sequence. This allows researchers to...Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed Repair Is Used for...Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything...

... DNA sequence called the Fertility factor (F-factor). The F-factor is found on an episome, a piece of ... information on viral vectors, including transduction download our Viral Vectors 101 eBook. Bacterial ...fragmented DNA released into the environment. Various factors promote natural transformation in different bacteria...bacterial or eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to ...scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage and plasmid properties...

...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website... the blog originally started as a way to easily store and share in-depth answers to frequently asked questions...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...informative resources that answer questions our depositors and customers are asking. So how can you get ...

...reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer than ...Kinetics of photon generation Slower Faster Cofactors/substrates Required Not required Signal strength...engineered Peak emission (nm) Size (kDa) Substrate Cofactors Secreted North American firefly (Photinus pyralis...AkaLumine ATP No Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...expression analysis to investigate the effect of regulatory elements — such as promoters, enhancers, or untranslated...untranslated regions — on gene expression. The regulatory element is cloned upstream of the luciferase ...energy from an excited donor fluorophore to an acceptor fluorophore, which then emits it as light. Bioluminescence...

...contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes taking place...their funding should also be published. All these factors will influence your choice of journals to submit...communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research and the CARE ... are two sets of guidelines frequently used by editors. Providing all this information makes your manuscript...who the reviewers are). If the reviewers and the editor decide that your manuscript needs to be revised...the conclusions of your paper. This will help the editor handling your manuscript decide on whether your... is appropriate for that journal. Argue with an editor or reviewer over their decision. If you do object...

...proteins, such as human growth factor, vascular endothelial growth factor, and interleukin-6 (Witkiewicz... was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "Fábrica Centro...myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal... in increased expression of transforming growth factor-β signaling, possibly inducing the secretion of...seems to attack us by releasing mitogenic growth mediators and improving cancer cells proliferation.  How...enzymes and metabolism reprogramming aren’t the whole story. It’s essential for a tumor to spread and to resist... Pinho! Bárbara Pinho is currently a science communicator at the Portuguese science museum "Fábrica Centro...