We narrowed to 997 results for: lars;

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...workshop. These workshops illustrate the open and modular nature of the workshop content: different parts...results, and tracking material downloads. This was particularly helpful for securing funding to support this...

...basics of molecular biology and cloning, since they have reasonable introductions to lots of molecular biology...quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr was the hottest phone...biology,” — Reddit commentor Yay4sean, in r/molecularbiology.   Oh, to be young again! Addgenie Marcy...ready-to-use viral vectors from some of our most popular lentiviral and AAV vectors, as well as developing...

...academic lab, a similar situation could arise if a lab veteran who worked with a particular sample suddenly... lab management software aren’t just limited to large volume facilities; it can be useful in academic ...you sad),  someone has put in a request for a particular plasmid and now it’s time for our lab technicians...this, it is important to write loosely coupled, modular code. Use tried-and-true tools We use Python, Django...

...these mutants are not toxic to HEK cells and that similar mutations can improve the specificity of Cas9 derived...from S. aureus. This means you can likely apply similar rational mutations to your Cas9 homolog of choice...SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken sequence independent...found in eSpCas9, the effect on specificity was similar; SpCas9-HF1 generated fewer off-target cuts when...variant displays comparable on-target activity and similar or decreased off-target activity across the sites...

...as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental...and CFP can activate certain T-DDOG constructs similarly to GFP, but commonly used red fluorescent proteins...activating gene expression. Although Cre isn’t a modular protein, Tang et. al were able to create a split...to many types of proteins. For proteins with a modular structure, constructing split variants should be... also possible to create split versions of non-modular proteins like Cre. Could Cas9-DOG be developed ...

...perform more modern techniques used in genetics or molecular biology because the experiments are either not...templates which they can easily generate off of a circular plasmid via PCR. Typically, to make these constructs...complementary DNA (cDNA), and then use PCR and molecular cloning to insert the cDNA into the desired plasmid...those who are interested in performing their own molecular cloning and investigating genes not in this collection...vector backbone. The empty vector PLL4, which is similar to PR-T4P, is available from Addgene. If you use...

... the digital music revolution hit. I predict a similar revolution for antibodies. Recombinant antibody...that may not work well in animals because of similarities to host proteins. After the antibody of interest...long-term stability, consistency between batches, and molecular definition of recombinant antibodies are essential...US alone (Bradbury & Plückthun, 2015a). With molecularly defined antibodies that do not change over time..., Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies are...

...friendly, to own the process entirely remains a large undertaking. Priority number one was to ensure that... There are regions of some plasmids that are particularly difficult to sequence and assemble, including...regions that are GC rich. Since NGS assembles a larger sequence from many smaller sequences, regions with...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are...Viral preps and pooled libraries In addition to regular plasmid sequencing, many of you will be familiar...

...the bait at a particular point in time, i.e. when the bait is interacting with a particular protein partner...specifically identify proteins that interact with a particular protein  complex as opposed to an individual ...partner or when the bait is part of a particular protein complex. Not performed in a native cell context...bait proteins stop interacting. This feature is particularly useful for in-depth characterization of dynamic...

...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...that alters or eliminates the expression of a particular gene. Targeting vectors used to create knockout... a way to track successfully engineered cells. Similar to transgenic mice, knockout mice are engineered...off-target effects on regions of the genome with similar homology to the site of interest or create mosaic...recombination of floxed sites in the cell can occur. Similar systems include tetracycline or doxycycline mediated...