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We narrowed to 971 results for: lars;

Showing: 451 - 460 of 971 results

  1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...enhancer it targets is a “pillar of function” for our stem cells. Remove this pillar and the ESC dies. Order...knowledge of which stretches of the genome are pillars for stem cells, or metastasized tumor cells, or... a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional globule of ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

  2. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function, they found that...found two that display robust cleavage activity similar to that of Cas9. These two nucleases, AsCpf1 and...respectively), both cleave in a staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9.... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

  3. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... and DNA repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...adenine deaminase TadA.   After seven rounds of molecular evolution, they obtained four adenine base editors...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...10.1038/s41587-020-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R.,...T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Evolution...

  4. Hot Plasmids - August 2020

    Type
    Blog Post
    ...from purified recombinant proteins at small and large scale.  This new technique involves selection in...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...affinity purification required! The team used other molecular techniques to introduce time modulation of the... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...PHP.V1 that exhibits efficient transduction of vesicular brain cells. Find this AAV at Addgene New serotypes...

  5. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... pCas/pTargetF system has been one of the most popular genomic editing systems in E. coli. However, some... linearized pEcgRNA backbone can be prepared in large amounts and frozen for future use or directly ligated...Academy of Sciences (CAS) Center for Excellence in Molecular Plant Sciences in 2019. She joined Sichuan Normal... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences since December 2020. His research... Yang Lab of the CAS Center for Excellence in Molecular Plant Sciences in September 2019 as a Ph.D. candidate...

  6. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...chalk talk is effectively a million dollar sales pitch. Given this large sum, one might expect that applicants...accept you as a colleague and give you a million dollar startup package. There are three steps to chalk...resources required to feasibly accomplish the aims and clarify what type of aim you are describing. It's easy ...Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from Oregon Health...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...complex approaches to scientific questions become popular. There are only so many methods and machines one...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... talking to them at a seminars, or even set up regular meetings. Meeting people outside your organization...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

  8. Viral Production at Addgene

    Type
    Blog Post
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination ...silver staining or SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid...lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a...virus protocols. Browse our plasmid collection of popular lentiviral vectors. This article was written by...

  9. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...restriction enzyme cloning. Instead, you can choose a molecular cloning technique that will work well with a given...1990s, Gateway cloning technology has become very popular as a rapid and highly efficient way to move DNA...has ready-made entry clones available for many popular genes, including Hs.KRAS4a. Use our website to ...Addgene’s ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables...Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  10. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... are just 2 examples of organizations that hold large, annual conferences. Gordon conferences offer more...young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face... Career Expo and the ACS career fair, which are larger meetings offering resources for students seeking...getting a phone interview! If you don’t have a particular employer in mind, you may consider starting with...pages. Make yourself visible on LinkedIn and other popular job site webpages. Importantly, find a good mentor...
Showing: 451 - 460 of 971 results