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  2. An Addgene Summer Internship

    Type
    Blog Post
    ...Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ...

  3. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...worthless. But simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts.... Practice writing everyday Overall, the most important instruction the classes taught me was to write...

  4. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ... Society, or maybe you think about a local food pantry or community arts organization. Many people are...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...

  5. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...Experiments Experiment  Detection Method Notes Quantification of a process Plate-based assays (plate reader... HPLC (proteins or other molecules) Isolate or quantify cells, proteins or other molecules that have been...

  7. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...different cellular responses. One of the biggest advantages of such a tool is being able to identify specific...screening technique. Lastly, the activity of both antagonists and inverse-agonists could be measured using ...

  8. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...two inverted terminal repeats (ITRs); but when recombinant AAV (rAAV) is made in the lab, rep and cap are...2020) Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction. Human ...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...chemistry degrees and provide advice to students who want to study chemistry. The society currently has 352...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that...

  12. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...

  14. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development...

  15. Intro to Our Summer SciComm Series!

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    Blog Post
    ...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly!  The question   The first, and most important question, you should ask for any science communication...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...that produce useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously...mCherry and GFP expression to check for ABE’s. b) Quantification of base-editing efficiency using XMAS-TREE-...

  18. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...
Showing: 901 - 920 of 1080 results