We narrowed to 958 results for: ARC

1. Viral Vectors 101: Voltage Indicators

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   ...ability to monitor their signals – electricity! The research tools for studying voltage within a cell are genetically...

2. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...out Addgene's virus protocols Find AAV for your research ...

3. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...with either blasticidin or puromycin selection. Researchers at the Allen Institute for Cell Science recently...

4. Cloning Mammalian Cells with the Agarose Method

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   ... biological screens. For example, for cancer researchers, if growth properties in agarose are of interest...

5. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...function of a protein or its role in a disease, researchers often isolate proteins of interest and examine...

6. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   ...exclusively because historical studies of pathogen research have conclusively shown that barrier protocols...

7. Antibodies 101: Producing Recombinant Antibodies

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   .... Recombinant Antibodies in Basic Neuroscience Research. Curr Protoc Neurosci. 2020 Dec;94(1):e106. doi...

8. Plasmids 101: Secondary Nanobody Toolbox

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   ...you ready to start using nanobodies for your own research? Great! Pleiner et al. have done the hard work...

9. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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   ...your target epitope(s). To achieve these goals, researchers commonly use one of two types of fixatives - ...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...universally compatible with all bacteria used in research, it isn’t a widely popular selection agent. Pros...

11. DNA Purification Without a Kit

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    ...number of spin columns you need to complete your research. Do you know of other nucleic acid purification...

12. A Guide to Starting Your Own Journal Club

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    ... of learning about new cutting edge science, researchers of all varieties must stay current with literature...

13. Tips for Screening with Yeast Two Hybrid Systems

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    ...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

14. Cpf1: A New Tool for CRISPR Genome Editing

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    ...location and GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function,...

15. Plasmids 101: Protein tags

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    ...decades of trying to address these challenges, researchers have amassed a considerable molecular tool box...

16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...sequence. While developing the MoClo system, researchers wisely agreed on universally using the same four-base...

17. Plasmids 101: Stringent Regulation of Replication

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    ...initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar...

18. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    ...Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    ...modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, activate, ...