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Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids will ...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene ...
CRISPR-mediated Plant Base Editors

Type

Blog Post

... is an Ag-biotechnologist in the National Rice Research Institute, Cuttack, Odisha, India. Currently Kutubuddin...
Plasmids 101: Stringent Regulation of Replication

Type

Blog Post

...initiation of chromosome replication. Nucleic Acids Research, 46(12), 6140–6151. https://doi.org/10.1093/nar...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

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Blog Post

...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...or as fluorophores in several different ways. Researchers have used oligonucleotides in fluorescent visualization...
Plasmids 101: Gateway Cloning

Type

Blog Post

... genes, including Hs.KRAS4a. Use our website to search for your favorite gene! STEP 2: Generate an Expression...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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Blog Post

...modifications. Cas9 has been modified to enable researchers to knock out, knock in, base edit, activate, ...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All Things Lentivirus with our Lentiviral...
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

Type

Blog Post

...approaches developed in the early 2000’s which allow researchers to deliver oversized cargo (Hirsch et al., 2010...
The Fluorescent Vegetables in Aptamer Soup

Type

Blog Post

... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...
Plasmids 101: Biotinylation

Type

Blog Post

...ssDNA or RNA–protein interactions.” Nucleic Acids Research, 23.18 (1995): 3792–3793. PubMed PMID: 7479014...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...more to optogenetics than neuronal activity. Researchers have designed a variety of photoswitchable elements...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

... with Linkers (MODAL) strategy." Nucleic acids research 42.1 (2014): e7-e7. PubMed PMID: 24153110. PubMed...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...
Antibodies 101: The Basics of Western Blotting

Type

Blog Post

... that you can make on your own. To narrow your search, first pay attention to the validated applications...
Viral Vectors 101: AAV Serotypes and Tissue Tropism

Type

Blog Post

...numbered AAV1, AAV2, and so on. In addition, researchers have created many engineered serotypes by mixing...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...the (all-too-common) event that homology-based searches return no candidates. Our strategy: Express random...
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Type

Blog Post

...pretty amazing. Despite the shifting landscape of research opportunities, it’s an exciting time to be in ...
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Type

Blog Post

...blogger, Beth Kenkel Beth Kenkel is currently a research scientist in the Department of Laboratory Medicine...

Showing: 901 - 920 of 923 results

Results per page:

Plasmids 101: Plasmid Incompatibility

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

CRISPR-mediated Plant Base Editors

Plasmids 101: Stringent Regulation of Replication

CRISPR 101: Targeting RNA with Cas13a (C2c2)

Fluorescent Proteins 101: Aptamer Fluorophores

Plasmids 101: Gateway Cloning

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Getting the Most from Your Lentiviral Transduction

Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

The Fluorescent Vegetables in Aptamer Soup

Plasmids 101: Biotinylation

New Optogenetic Tools for Cytoskeleton and Membrane Control

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Antibodies 101: The Basics of Western Blotting

Viral Vectors 101: AAV Serotypes and Tissue Tropism

Technique: Probe Phage Genomes for Host Binding Proteins

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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