We narrowed to 957 results for: Cre
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TypeBlog Post... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...
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Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating... -
Lab Automation at Addgene
TypeBlog Post...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete... -
How to Prepare for an Industry Interview
TypeBlog Post...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ... -
Of Myc and Men
TypeBlog Post...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...Twitter in real life. If there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour... -
Academic vs. Industry Postdocs
TypeBlog Post...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics... -
10 Basic tips for mammalian cell culture
TypeBlog Post...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...starting vector that the depositing lab modified to create their new plasmid. The Addgene Vector Database... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...addition, there is a drop in image brightness with increasing depth caused by light scattering and absorption... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... the time it takes the FP to correctly fold and create the chromophore. This can be from a few minutes... -
An Introduction to Adenovirus
TypeBlog Post...makes sense that vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early... -
Getting to Know Expansion Microscopy
TypeBlog Post...don’t have to optically magnify quite so much. Created with biorender.com Steps 2-4 are not found in...