We narrowed to 962 results for: Cre

1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

2. Antibodies 101: Conjugation

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   ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

3. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...

5. Working with Nuclear Receptors

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   ... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

6. Lab Automation at Addgene

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   ...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...

7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating...

9. How to Prepare for an Industry Interview

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   ...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes...

11. Adapting Toehold Switches to Detect Zika Virus

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    ...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

12. Tips for Titering Your Lentiviral Preps

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    ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

13. Tips to Make the Most of a Scientific Conference

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    ...Twitter in real life. If there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour...

14. Academic vs. Industry Postdocs

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    ...were having a positive experience, I was still incredibly nervous about leaving academia. A lot of academics...

15. 10 Basic tips for mammalian cell culture

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    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

16. Viral Vectors 101: Producing Your rAAV

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    ...Golde, T. E. (2020). Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation...

17. Twenty Years of Addgene Sharing: CRISPR

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    ...17,560 CRISPR plasmids. That’s almost a ninety-fold increase in size! From this collection, we’ve shared almost...

18. Viral Vectors 101: Voltage Indicators

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    ...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ...

19. Of Myc and Men

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    ...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

20. Anatomy of a Plasmid Page at Addgene

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    ...starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector Database...