We narrowed to 967 results for: Cre

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   ...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...

3. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...

4. CRISPR 101: Cas9 vs. The Other Cas(s)

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   ...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...

5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...

6. Antibodies 101: Conjugation

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   ...protein-protein interactions with other peptides. Created with BioRender.com.     Many biologists can...

7. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

8. Visualizing Protein Turnover In Situ

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   ...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...

9. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...

10. Working with Nuclear Receptors

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    ... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...

11. Lab Automation at Addgene

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    ...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...

12. How to Prepare for an Industry Interview

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    ...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...

13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes...

14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...

15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating...

16. Adapting Toehold Switches to Detect Zika Virus

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    ...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

17. Tips for Titering Your Lentiviral Preps

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    ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

18. Tips to Make the Most of a Scientific Conference

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    ...Twitter in real life. If there is not a tweep up, create one yourself. Pick a time (ex: lunch, happy hour...

19. 10 Basic tips for mammalian cell culture

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    ...making their surrounding media acidic through the secretion of lactic acid (a by-product of cellular metabolism...

20. Viral Vectors 101: Voltage Indicators

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    ...hydrolyzes a precursor dye to make it more lipophilic, increasing its affinity for the membrane, where it will ...