...that are built into how something functions, were designed for this purpose. One such control is dividing...

... and to work in a variety of applications. The desired specificity of your secondary antibody depends ...

...v) HCl Adjust to pH 2.0–2.2 For the mildest stripping solution, omit the Tween 20. Once your...

...guanidine moiety at the end of its side chain. Decades ago, other labs discovered that the in vitro functions...

...for faster detection of RCA. 2nd Gen AdVs were designed to have increased cloning capacity, decreased  ...

...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps required...

...communication and learning to work with people despite the distance improved my verbal and written communication...

...Derek Jacoby from Makerspace Victoria, CA Over the decades science has become increasingly restricted to academic...

...familiar with the minute details of your work. Describe the problem by stating the facts and explain how...

...restriction enzyme digest (subcloning), including design and experimental procedures....easily move YGOI into a mammalian expression vector. Design (Choosing enzymes) Many DNA analysis tools, including..., but do not cut within your insert Are in the desired location in your recipient plasmid (usually in ...your recipient plasmid as well as a specifically designed test digest later to verify that the insert was... fear. You have other options, such as: Adding desired restriction sites to flank your insert : You can...restriction enzymes that cut within your insert. Adding desired restriction sites to your recipient plasmid : You... orientation of your insert, so you may want to design a diagnostic digest for this purpose. Congratulations...

...Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol describes how ...This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells...type will be able to grow under the conditions described in this protocol. Conditioned medium (details ... specific conditions used. Each 96-well plate described in this protocol could, in theory, give rise to...protocol was performed at Addgene on the A549 cells described in our sample data , each 96-well plate gave rise...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

...specific details about the individuals, each example provides a scenario where both people get something valuable...

...Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional...

...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...

... (about three to four days), this second round provides the added benefit of producing a stock P1 virus...

...Maria were encouraged by previous reports that described magnetic responses of cryptochrome proteins or...

...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers...

...investigators to identify specific clones with desirable properties, either initially, or using secondary...

...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...