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...of the contest is our traditional approach. You might decorate your lab in pipette tip snowflakes, hang...

...shape Addgene's future. Listen to this episode for insights into the process of getting an MD PhD, the inside...

...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

...plasmids shipped. Reaching one million plasmids shared might have you wondering how many plasmids leave Addgene...

...open to industry scientists. This collection is highly diverse in terms of target organisms (from bacteria...

...that readily diffuse through plant tissue. Those might be the most important to know about, anyway! Interested...

...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

... school for microbiology, and I ended up going straight to law school. This was in the early 80s, and ...

...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

...the labs they’re granted to, as well as shine a light on some of the great minds working in developing...

...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...

...community through sharing science and distributing high-quality materials and information. As a scientist...

...lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR...

... Find job listings at Addgene Get more career insight from Addgenies ...

...One Pot, One Step, Precision Cloning Method with High Throughput Capability." PLoS ONE. 3(11): e3647 (...

...little more certain that I was being led down the right path. The frequent Zoom meetings helped me communicate...

...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

...expression system. If you find that your protein might not fold well, you should screen a few different...

...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...