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We narrowed to 1,106 results for: Ide

Showing: 901 - 920 of 1106 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...interactome of these key regulatory molecules, and has provided clues to their previously uncharacterized cellular...acetyltransferase to luciferase, the basic assay remains widely used today.  The sheer abundance of these critical...greatly increased the burden associated with identifying plasmids in the research literature and acquiring...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...initiation step of gene transcription; today we'll provide an overview on how transcription stops, or termination...post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The...be detailed here, but additional references are provided at the end.  Nearly all common bacterial expression...

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...molecular tools to map neuronal connectivity are widely used in neuroscience. In this post, I’ll describe... in first order neurons, the G-deleted RABV can divide and replicate (since these processes are mediated...Another study used RABV monosynaptic tracing to identify inputs to two major dopaminergic cell populations...

  4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...specificity of AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that ...here.  Applications for synthetic promoter AAVs Besides restricting AAV transgene expression to particular...synthetic promoters with high rates of labeling are ideal of gene therapy applications, promoters that produce...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...below. Types of Voltage Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive...voltage sensing component (i.e., rhodopsin naturally resides in the membrane), or both. Targeting these systems...Addgene’s Virus Protocols Addgene’s Chemogenetics Guide Resources on the Addgene blog Viral Vectors ...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell...few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination of expression...Empty Backbones Check Out Our Fluorescent Protein Guide ...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...SpCas9. Hu et al. performed GUIDE-seq analysis to characterize genome-wide off-targets, since increasing... Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn...

  9. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...that popularity through the years.  Libraries are divided into different categories based on function: knockout... years, even as more CRISPR technologies become widely used and available. If we look closely at Figure...navigate that learning curve. As of 2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR ...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...some stage, then choosing a HaloTag could pay dividends down the road. It is difficult to predict precisely...

  11. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...factor of 2. This means that if you set up two identical assays with the same sample, you could end up ... should fall within the range of the assay for a wide range of titers. We usually dilute our samples 1...experiments.  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital...

  12. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...sites. FRT sites and loxP sites differ at the nucleotide level but share an overall structure of 13 base...37 °C). Smart molecular evolution led to the identification of FLPe which has a temperature optimum of ...Recombination between the two target sites with identical sequence leads to inversion of the flanked DNA...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... codon, stop codons, reading frame considerations, etc). This video demonstrates how to use the Snapgene...Protocols Watch Our Diagnostic Restriction Digest Video Check Out Our Molecular Biology Reference Pages  ...

  14. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...more funding and research opportunities are being provided to immunotherapy than ever before. Although different...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...(ADAO) are working toward such a ban, and the president of the organization, Linda Reinstein – whose husband...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...ssDNA is packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived...strand: A mix of degenerate primers and the high-fidelity Phusion polymerase are used to introduce point...

  16. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...fluorescence can occur. Cre recombinase can be used alongside common, existing tools for fly research that use...turnover of Langerhans cells in the dermis and identify that Langerhans cells are replenished through ...The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations." Proceedings...

  18. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated...on our website and blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series...

  19. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ...      And you’ll be able to find all published videos in our Quick Tips playlist:  Want your quick tips...be sharing our favorite parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube...

  20. Help Us Name Blugene’s New Virus Friend

    Type
    Blog Post
    ...your favorite name. We hope you submit your best ideas because the finalists will receive some newly designed...Learn more about Blugene's make-over Get a how-to guide on the new Addgene search engine ...
Showing: 901 - 920 of 1106 results