We narrowed to 937 results for: KIN

1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   ...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...

2. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   ...gene in the recipient's genome. Antibiotic plates lacking DAP select against donor bacteria and for recipients...

3. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   ...were the only realistic options for scientists working in regenerative medicine, but the longing for a...

4. Plasmids 101: Protein Expression

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   ...antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems Yeast are a great ...

5. Plasmids 101: Degron Tags

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   ...round of evolution (AID-2) to reduce some of the leakiness associated with the system, but the general concept...

6. PCR Applications for Copy Number Variation Assays

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   ...relative quantification - that is, since you’re looking at the amount of DNA in your sample relative to...

7. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... experiments. Considerable effort has gone into making FPs monomeric, yet many investigators remain ignorant...

8. Anatomy of a Plasmid Page at Addgene

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   ...found yourself bamboozled by all of the different kinds of information on our plasmid pages? Well, to help...

9. Tips for arabidopsis transformation

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   ...phenotypic analysis it is appropriate to begin working in the T2 generation. The selection process itself...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... Addgene Blog Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the ...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...

12. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...

13. Great Results Start with Great Standard Curves

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    ...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

14. An Introduction to Adenovirus

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    ...resources References Bauzon, M., Castro, D., Karr, M., Hawkins, L. K., & Hermiston, T. W. (2003). Multigene expression...

15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...been limited, in part, due to the necessity for working with this virus under the highest biosafety level...

16. How to Write a Scientific Review Article

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    ...So, as long as it doesn't look done, just keep working on it. Also bear in mind that this is just a review...

17. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single base editing efficiency...

18. CRISPR-mediated Plant Base Editors

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    ... repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or reverted...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

20. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...