We narrowed to 1,016 results for: LEA

...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Restriction Enzyme Cloning Restriction enzyme (endonuclease) molecular cloning is the "classic" cloning ...naturally produced by certain bacteria and archaea, cleave double-stranded DNA (dsDNA) at specific sequence...four to 13 base pairs, and produce predictable cleaved ends in the DNA fragments. Given its prevalence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...

...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...

...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...