We narrowed to 1,018 results for: LEA

1. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   ...collection.   Check out the SeqWell website to learn more about our plexWell kits and services! Many ...

2. FPbase: A new community-editable fluorescent protein database

   Type

   Blog Post

   ...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

3. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   ...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

4. Choosing Your Fluorescent Proteins for Multi-Color Imaging

   Type

   Blog Post

   ...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

5. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   ...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

6. Viral Vectors 101: Viral Vector Elements

   Type

   Blog Post

   ...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

7. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   ...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

8. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   ...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   ...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

10. Plan Your Experiment

    Type

    Guide

    ...targets may not result in equivalent cleavage efficiency. In fact, cleavage efficiency may increase or decrease...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...decrease expression of a target gene? Once you have a clear understanding of your experimental goal, you are...also important to consider factors that maximize cleavage of the desired target sequence, or on-target activity...exhibits the lowest immunogenicity and are one of the least toxic methods for in vivo viral delivery. Additionally...Before proceeding, we recommend asking labmates/colleagues, searching the literature, or discussing with...Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and gRNA. Before...

11. Addgene: The First Twenty Years

    Type

    Blog Post

    ...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

12. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    ...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

13. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    ...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

14. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

16. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

17. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

18. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

19. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

20. Antibody Guide

    Type

    Guide

    ...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...