...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists...

...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...

...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

...description of 2nd and 3rd generation lentiviruses, please review 2nd vs 3rd generation above . Briefly, 2nd...Ther. 2005. 5(4): 387-398. PubMed . HIV-1 Genome Nuclear Import Is Mediated by a Central DNA Flap. Zennou...and partially spliced transcripts to facilitate nuclear export. Tat in trans Second generation only; Trans-activator...stimulates the expression of transgenes via increased nuclear export. LTR Sub-components: U3 R Sub-element: TAR...

...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...