We narrowed to 1,027 results for: LEA

1. Antibodies 101: Epitope Tags

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   Blog Post

   ...suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out Addgene’s monoclonal...

2. Antibodies 101: Chimeric Antibodies

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   ...antibodies may be the right solution for you. Read on to learn more. What is an isotype? Antibodies are composed...

3. Viral Vectors 101: Transductions

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   Blog Post

   ...genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of your choice...

4. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ...catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable single ...

5. Reagent Repositories Are Speeding up Science During the Pandemic

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   ...Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids to all scientists...

6. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   ...the best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps ...

7. The Power Behind NGS Plasmid Validation: seqWell

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   ...collection.   Check out the SeqWell website to learn more about our plexWell kits and services! Many ...

8. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

   ... the preprint repository BioRxiv. This example clearly illustrates the power of collaboration, which despite...

9. FPbase: A new community-editable fluorescent protein database

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   ...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

10. Resume Writing for Non-academic Science Careers

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    ...what you are capable of. You would be surprised to learn how often I see resumes from good candidates that...

11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    ...most detectable and so should be used to tag your least abundant proteins, with the blue and infrared channels...

12. Molecular Cloning Techniques

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    Guide

    ...properties of type IIS restriction endonucleases. These endonucleases cut dsDNA at a specified distance...' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...Restriction Enzyme Cloning Restriction enzyme (endonuclease) molecular cloning is the "classic" cloning ...naturally produced by certain bacteria and archaea, cleave double-stranded DNA (dsDNA) at specific sequence...four to 13 base pairs, and produce predictable cleaved ends in the DNA fragments. Given its prevalence...cloning project. Restriction enzyme cloning also leaves behind a short scar in the DNA sequence and can...

13. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...both a screen and a selection, it is important to clearly define what type of factor you are looking for ...

14. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post

    ...techniques and troubleshooting methodologies drew me to learn more about the process that was used to create this...

15. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...protein that helps with virion assembly and particle release from late endosomes when AAV is trafficked. Rep...

16. Antibodies 101: Flow Cytometry Controls

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    Blog Post

    ...should have a FMO sample for any marker without a clear positive and negative population. If you’re unsure...

17. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    Blog Post

    ...Cloning Techniques like SLIC and Golden Gate Cloning Learn How to Verify Your Plasmid Read our Plasmids 101...

18. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

19. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

    ...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

20. Addgene: The First Twenty Years

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    Blog Post

    ...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...