We narrowed to 992 results for: Ran

1. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   ...increase your competitive edge, they alone, are no guarantee of success. Don’t luck into an internship, prepare...

2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   ...information (more on this below). Plating the transformation on ampicillin plates containing X-gal allows...

3. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   ...across a number of different sites. Using a combined random and directed mutagenesis approach, the Anna Cereseto...

4. Clear and Accessible Writing

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   Blog Post

   ...resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts...

5. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ...spilling over" into another channel's detection range. Though you can compensate for this when reading...

6. Improved Plasmid Maps Powered by SnapGene

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   Blog Post

   ...in-house software and also eliminates many of the extraneous ORFs that cluttered our old plasmid maps. Clearly...

7. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   ...experiments and to find tools that will make your transition into the field much easier! If there’s a plasmid...

8. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...P, Frenzel A, Wong J, Koh XY, Eng H-Y, Lane D, Lefranc M-P, Clark M, Dübel S (2018) When monoclonal antibodies...

9. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ... to research potential future employers and to arrange informational interviews. 4. “Tips for Scientists...

10. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ...like a wet lab experiment I use the text editor TextWrangler to record the following, which are later printed...

11. Dos & Don'ts When Publishing a Scientific Manuscript

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    Blog Post

    ...full of typos. Don’ts: Submit your manuscript to random journals or as many journals as possible at the...

12. Lentivirus ddPCR Titration

    Type

    Protocol

    ... is based on an LV titer range of 1E+05–1E+09 TU/mL, where TU is transducing units and generally represents...July 7, 2023 Workflow Timeline Day 1: Seed and transduce cells Day 4: Treat cells with Benzonase and harvest...Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare the following... pipette 10–20 times. Use a new pipette tip to transfer 200 µL of the first dilution into the next dilution... plate (1 dilution per well). Leave one well untransduced (add 150 µL of DMEM complete instead of a viral... into the wells containing the virus and the untransduced control. Pro-Tip For even seeding, prepare a...min. Resuspend cells in 500 µL DMEM complete and transfer to a microcentrifuge tube. Centrifuge for 5 min...

13. Summer SciComm Series: Cognitive Load

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    Blog Post

    ... the effort associated with a specific topic; extraneous cognitive load refers to the way information ...

14. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    ...in light green; and proteins are represented in orange. Created with BioRender.com.    How do secondary...

15. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    .... “I was lucky to have been able to make that transition really smoothly,” says Susan. After being in ...

16. The time and cost required to make a plasmid

    Type

    Blog Post

    ...relevant work in your field! However, there’s no guarantee you’ll get a response on the first try (or any...

17. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    ...results, but this result shouldn’t be taken for granted for all research going forward. It’s just not that...

18. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    ...vector + insert may spontaneously religate, this transient construct retains functional Type IIS sites and...

19. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...your area. When you are in ‘writing-mode’ for a grant or a scientific manuscript, the reading will likely...

20. What is Polymerase Chain Reaction (PCR)

    Type

    Protocol

    ...step heats the double stranded DNA template strand to the point where the strands start denaturing and ...this temperature range to anneal to each of the single stranded DNA template strands. The DNA polymerase...of the sense and anti-sense strands of the template DNA. They are strands of nucleic acid that are starting...molar ratio. It also requires two unique single stranded DNA oligonucleotide (oligo) primers, which anneal...seconds at 94°C: Continued denaturation of double stranded DNA. Anneal primers for 30 seconds at 55°C: The...polymerase to synthesize and elongate the new target DNA strand accurately and rapidly. Repeat steps 2-4 25-30 ...fill-in any protruding ends of the newly synthesized strands. Materials List Reagents for each 50µL PCR: Thin-walled...