We narrowed to 1,045 results for: STI

...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

...limited to restriction enzymes - although many labs still love using them! With so many technologies currently...an ordered set of scarless ligations following digestion with Type II restriction enzymes. All of these...

...cross-linked, DNA is digested into smaller pieces. Sticky ends created are then ligated together to create...interactions opens the door for answering more questions about chromatin conformations. The Xu lab presented...

...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

...proteins, it’s important to note that despite their distinction by name, chromoproteins are actually a subset...wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to ...

... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

...easier because individual labs will not need to ‘domesticate’ all the basic and necessary elements in order...purification; the whole cloning process is so simplistic once you have your parts. That was the motivation...

...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

...genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...

...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...studies), as well as translational applications (mechanistic studies and gene therapy treatments for neurological...

...viral vector production facility, food allergy testing lab, and the grad student down the hall from you...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...

...PMCID: PMC5915492. Witte, Anna Kristina, et al. "A systematic investigation of parameters influencing droplet...

...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...

... one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation, the original...and XhoI MluI BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional...

...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells... for cancer immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers...

...precautions, these systems allow researchers to investigate viral processes in extreme detail. The mechanism...these components are necessary to efficiently reconstitute a functional viral polymerase complex and utilize...

...expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre recombinase. FLPe codon ...Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate billions ...

...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...

... tag antibody produced by our partners at the Institute for Protein Innovation (IPI), part of a new collection... more highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged...