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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: STI

Showing: 901 - 920 of 1045 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...mammalian cell expression, and compatible with constitutive or Cre-dependent expression. These sensors have...the calcium sensor to specific tissues or even distinct cell subtypes for efficient expression and downstream...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...prime editing in both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to...activation kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option...

  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...transfection reagents and keep the cells happy. Harvesting virus Once you have transfected your cells, wait...non-genome-containing (empty) capsids, thus leading to an overestimate of transducible units in the virus preparation...

  8. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... lead to a wealth of additional experiments investigating the role of individual genes or entire pathways... you use them for! A well-developed biological question and experimental system are absolutely necessary...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Therefore, FPs are used to study many biological questions. The two most common uses are: 1) to test the ...microscopes the filters are not narrow enough to distinguish between closely related colors. Furthermore, ...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy)? AAV...Additionally, AAVs do not integrate in the host genome but still lead to long-term (>10 years) transgene expression...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...secondary nanobody, it’s important to review a few characteristics of your primary antibody: species it was raised...subclass. Once you know the answer to these two questions, refer to this table to find the right nanobody...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...labeling is to inject a mix of AAV vectors that constitutively express a red, green, or blue (RGB) fluorescent...studies), as well as translational applications (mechanistic studies and gene therapy treatments for neurological...

  14. MXS Chaining

    Type
    Blog Post
    ... one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation, the original...and XhoI MluI BioBricks SpeI and XbaI EcoRI and PstI Bglbricks BglII and BamHI EcoRI Additional...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...viral vector production facility, food allergy testing lab, and the grad student down the hall from you...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...

  17. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env, which can only infect CD4+ cells... for cancer immunotherapy, but this research is still in the pre-clinical/early clinical stages. Researchers...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...
Showing: 901 - 920 of 1045 results