We narrowed to 1,049 results for: URE

...the plan of being a scientist. I pretty quickly figured out that I just don’t have the patience to be a...Career Channel of the blog so you don’t miss out on future career posts! Additional Resources on the Addgene...

...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ...gene expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576...

...reporter output was observed in their 293 cell culture system. In cells cotransfected with GFP and the...types of proteins. For proteins with a modular structure, constructing split variants should be relatively...

...spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed a fusion of keratin...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...

...Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst among...only assist you and make you more qualified for future career opportunities. Becoming involved is not ...

..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...

...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

... 350(13): p. 1353-6. PubMed PMID: 14999088. 7. Turetsky, T., et al., Laser-assisted derivation of human...cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663...

...thousands of investigators studying them, how do we ensure that the research community has access to all of...24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID: 25877186...

...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

... two sgRNAs isn’t feasible for your experiment, ensure your sgRNA sequence has little homology to other...mediates efficient homology-directed repair and temperature-controlled genome editing. Nat. Commun. 2017 ...

... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ...in mice. Expression from the hybrid vector, as measured by β-galactosidase activity, was 81% of that from...

...-based applications in your experiment...but not sure where to start or what your options are? Addgene's...

...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

...milestones Addgene is poised to reach in the near future! We’re almost at:  100,000 different plasmids ...