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We narrowed to 987 results for: des

Showing: 901 - 920 of 987 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...to a lay audience. My lay paragraph typically includes a couple of sentences of introduction to my area...

  8. Gibson Assembly Protocol

    Type
    Protocol
    ... Procedure Design your plasmid and order primers (see figure to the right). When designing your plasmid...and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance gene...and yield. If there are significant amounts of undesired product, gel-purify DNA segments. Otherwise, PCR... High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed DNA fragments... (<150 bp). Pro-Tip Please note that the way to design the “stitching” primers and the amounts of primers...window) . NEB has other resources, such as a primer design tool (Link opens in a new window) . Gibson Assembly...

  9. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...There are four biosafety levels. This protocol provides information for both biosafety level 1 (BSL-1)...level has different safety requirements. BSL-1 is designated for those working with microbes that don’t cause...Staphylococcus aureus or Vibrio cholerae . BSL-2 includes all of the precautions needed in BSL-1, along ...before and after working in the lab. Ensure that a designated chemical waste accumulation site is present, ...(MSDS). While You Work Dispose of sharps in a designated sharps container. Sharps include anything that... drain. Solid BSL-2 waste can be collected in designated biohazardous waste containers that can be autoclaved...important! Disclaimer: The SOP presented here has been designed by the Addgene nonprofit plasmid repository and...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...created. Additional design rules can be found at the Broad Institute's TRC shRNA Design Process . To minimize...Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal...sites in place of the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence....9kb stuffer, visit www.addgene.org/10878/ . Description Vector Element U6 Human U6 promoter drives RNA...the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector ...website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal...accessed after a free registration. Guidelines for designing siRNAs with effective gene silencing: Starting...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    ...specific details about the individuals, each example provides a scenario where both people get something valuable...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Optimized dicot prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants,...

  17. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...
Showing: 901 - 920 of 987 results