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We narrowed to 938 results for: Kin

Showing: 461 - 470 of 938 results

  1. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study stem ...While many barcoding libraries are available for tracking cell lineage, different applications require careful... can transduce neurons for retrograde labeling, making it a powerful tool for circuit tracing and other...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...Whether you’re new to in-house virus production or looking to improve your current methods, understanding ... add an additional viral passage. This involves taking your first round of virus, called P1 (or sometimes... and may need to be optimized for your purpose. Making your decision The final decision of which system...10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment...

  3. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...Hi-C (Han et al., 2018). Think of 3C methods as taking a snapshot of chromatin looping with the photo ...CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions, a restriction...together to create loops of DNA. This is key to linking pieces of DNA that are far away from each other...Zhang, Y., Liu, Y., Liu, N., Botten, G.A., Cao, H., Orkin, S.H., Zhang, M.Q., & Xu, J. (2020). Multiplexed...

  4. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...Terskikh et al.) and even displays predictable kinetics over 14 hours of expression in C. elegans embryos...Miyatsuka et al.) Distinguish between models for trafficking to the lysosome by fusing Medium-FT to LAMP-2A...structure and a particular biological event (trafficking to a subcellular location, start of gene expression...change color from blue to red report on cellular trafficking." Nature chemical biology 5.2 (2009): 118-126...

  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...editing!  CRISPR functions in use But exactly what kind of editing are people doing with all these CRISPR... modifier. We used these categories to see what kinds of CRISPR tools people were depositing and requesting... share useful tools, and as such, we’re always looking to understand our community better. We therefore...year, or approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms...

  6. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA binding protein...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can be reprogrammed...potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance, when...PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin mice for genome editing and cancer modeling." Cell...

  7. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful... see if your cloning experiment worked just by looking at the cells on the plate. For example, if your... identification is a good place to start before taking on more time consuming and expensive methods. While...Learn more about FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find...

  8. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...enter industry since I was drawn to the idea of working as a team to develop therapies that could directly... since you can’t afford to spend too much time working on something extremely risky or exploratory. Getting...it does not divulge any propriety information. Working as a team: Industry is all about teamwork. A single...environment, surrounded by lab members who are all working on related topics, scientific discussion is often...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... unknown, it is entirely possible that you are working with a low copy plasmid. When increasing the culture..., or a home-brew optimized for plasmid yields. Spiking your media with supplements such as magnesium salts...can optimize your growth time, temperature, and shaking speed to maximize cell density and plasmid yield...especially those in deep-well plates, require a faster shaking speed for proper aeration (260-300 RPM). 37℃ doesn...

  10. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...Institute and Harvard Medical School. Scientists making transgenic organisms with Cas9 should be aware ...should be conducted with extreme caution. Why does making transgenic organisms with Cas9 risk creating a ...and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create an RNA-guided...mosquito species. Reproductive confinement involves working with laboratory organisms that can't reproduce ...
Showing: 461 - 470 of 938 results