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We narrowed to 971 results for: lars;

Showing: 461 - 470 of 971 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    ..., which is not well protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs...related voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...

  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...out.  In some fields, a cover letter is vital, particularly in fields where communication and writing are...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...working for that organization or group. This is particularly important for labs and nonprofits, who tend ... did, and state that it would help you manage a large number of files as part of the job. Structure of.... It can also convey your excitement for that particular job, your commitment to the organization's mission...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...often used to study the biology surrounding a particular gene (although it has been quite successfully...mutation and need to focus your attention to that particular region. No matter where you are looking, you ...the individual gRNAs in their PX330 plasmids (or similar plasmids for gRNA expression), one gRNA at a time...Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.... 3. Mashiko, Daisuke, et al. "Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas ...

  4. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...research. Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...test chamber is used in which a nose-poke at a particular hole (the active hole) induces laser stimulation...without Chr2. c-Fos is a reliable and widely used molecular marker of neuronal activation and is a sufficient... happens. You never know how stimulation of a particular brain region may affect the animal; stimulation...

  5. Using a Light Microscope Protocol

    Type
    Protocol
    ... your ocular lens. For example, if you observe something using the 10x objective and your ocular lens ...transmission electron microscopes that allow us to see cellular components with science-fiction levels of detail...are magnification (the ability to make an image larger) and resolution (the ability to distinguish between...Lenses Focus Knobs (Fine and Coarse) Nosepiece Arm Ocular Lens (Eyepiece) The route the light follows from...objective lens which magnifies the image, then the ocular lens, where the image is further magnified before...objective magnifies it 10 times, and so on. The ocular lens also provides magnification and the power ...diaphragm and use the coarse focus adjustment knob (the larger of the two focus knobs) until you can see the edges...

  6. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...landmark discovery in the 1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker...blog, we’ll be focusing on shRNA, one of the most popular choices for gene silencing. RNA interference RNAi...degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/10.1016/j.... and assessment of gene knockdown. Methods in Molecular Biology, 139–156. https://doi.org/10.1007/978-...on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology Reference ...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...arms between 500-1000 bps. HDR efficiency with circular plasmid templates is generally low; to increase...cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed. The Road Less ...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, harnessed these attributes of ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...as connections between motor neurons and the neuromuscular junction. In zebrafish, the method has become...neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods...

  10. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...cumate-inducible gene expression system, which functions in a similar manner to the Tet system. The transactivator configuration...interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed... binary systems above if they all behave very similarly?” The simple answer is that sometimes it is necessary...systems. Recent work has even used all 3 in a single modular vector for ease of use! Conclusion This post contains...
Showing: 461 - 470 of 971 results