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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 992 results for: Ran

Showing: 921 - 940 of 992 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ... Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation should be...of 5 mL (3 mL SOC + 2 mL transformed Endura from two separate transformations). Pro-Tip Do not pipette...assumes familiarity with standard bacterial transformation and basic knowledge of the library being amplified...amplified. Please see our protocol on Bacterial Transformation . The generalized pooled library amplification...premixed LB (VWR 101414-072) for convenience but any brand or house- made LB that supports normal growth is...using a new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes...repeatedly or mix when removing SOC containing transformed DNA-Endura from cuvette. Shake four 14 mL Vented...

  2. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...incubation, transform DH5α E. coli cells by heatshock with the plasmid of interest. See our transformation page...for a detailed heatshock transformation protocol. Plate 50 µL of transformed E. coli /rescue media suspension...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...plating antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of antibiotic makes...investigator to conveniently plate and select transformed cells containing plasmids differing in their ... sterile water (or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin (...carbenicillin, plated over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on ...

  3. A Look at Addgene's QC Process

    Type
    Blog Post
    ...accuracy across all of our plasmids and provide transparency in sequence discrepancies. While Addgene’s QC...

  9. Tips for Technical Support Calls

    Type
    Blog Post
    ...probably difficult for you to ascertain where on this range your question falls. Here are some things to keep...

  11. Of Myc and Men

    Type
    Blog Post
    ...an amide group added to its C-terminus post-translationally, which turned out to be critical for antibody...

  12. PhD Applications After COVID

    Type
    Blog Post
    ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...
Showing: 921 - 940 of 992 results