We narrowed to 1,085 results for: ANT

1. Science Careers: Unruly Interests Feed Many Paths

   Type

   Blog Post

   ... and data with authors and referees. And I am constantly teaching – often explaining a technical piece...focuses at Science on developmental neurobiology and plant sciences, viewed through the lens of development...

2. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

   Type

   Blog Post

   ...were known to be diversity hotspots containing abundant biological dark matter and so the lab organized...application from the publication is the removal of unwanted cDNA derived from 16S rRNA in bacterial RNA-Seq...

3. Donations from Addgene to Yield Answers for Rare Disease Researchers

   Type

   Blog Post

   ...Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma... with CRISPR-Cas9. There are so many different variants and strategies out there. This will help us acquire...

4. Another Pathway into Cells: iTOP

   Type

   Blog Post

   ... transduction? Niels Geijsen’s group in Utrecht wanted to pursue a nonviral strategy for transducing primary...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that...

5. Extracurricular Activities for a Strong Science Career Path

   Type

   Blog Post

   ...to publication and acquisition of funding. If a grant committee is not convinced that your project is ...getting a much-needed break from your lab bench.  Want further information about shaping your career to...

6. Plasmids 101: Gram Negative and Positive Bacteria

   Type

   Blog Post

   ...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

7. 25 Work From Home Tips for Scientists

   Type

   Blog Post

   ...calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ...

8. Visualizing Genomic Loci with CRISPR-Sirius

   Type

   Blog Post

   ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-...

9. Multiplexed Overexpression of Regulatory Factors (MORF) collection

   Type

   Blog Post

   ...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...

10. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    ...while still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered...

11. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

    ...his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...

12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    ...circular DNA takes on several conformations the most abundant being: supercoiled, relaxed and nicked. If your...your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA will make it difficult...

13. New and Upcoming Viral Vectors - May 2020

    Type

    Blog Post

    ...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

14. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ... genome-wide screening,” Konermann said. “If you want to activate all 20,000 genes, it is not possible...genes that, when turned on, make melanoma cells resistant to treatment with a BRAF inhibitor. (The test ...

15. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    ...for your audience. I ask myself that question constantly (and so should you!)  Amount of information and... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

16. Celebrating 15 Years of Scientific Sharing

    Type

    Blog Post

    ...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...

17. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

18. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    ...preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...

19. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    ...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

20. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...