We narrowed to 1,081 results for: ANT

...Modifying gene expression Generally speaking, one may want to achieve either inhibition or activation of neurons...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol...

... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus...

...plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are...

...CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and... (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of...

...18px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ... for reviewing this post! Dr. Ben-Simon is an assistant investigator at the Allen Institute in the department...

...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...

... Protocols DNA Quantification DNA Quantification You may also like... Addgene’s DNA Quantification Protocol. Protocols... different stages of molecular cloning, it is important to get a quick and accurate reading of DNA concentration...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...

...adapted for any antibiotic through the use of a selection curve to determine optimal antibiotic concentration...Learn how to add antibiotic to a plate after it's already been poured and solidified. Protocols...Streaking Bacteria on LB Agar Plate Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates...This protocol describes methodology for plating antibiotic over-agar for the selection of transformed E....E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate and... does not need to prepare separate batches of antibiotic-containing agar. This protocol will focus specifically...the protocol video below to learn how to spread antibiotic over-agar. Equipment Pipette tips for both pipetting...

...software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific...Collection Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs and When to Use Them...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide Introduction This protocol...Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody incubation...primary antibody and an isotype control antibody to the desired concentrations in the antibody dilution... with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary antibody for 2 ... a purified antigen to demonstrate the expected antibody dose response. Protocols...

...don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia...

...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

...all published videos in our Quick Tips playlist:  Want your quick tips even quicker? We’ll be sharing our...

...sweaters, Flannel Fridays, Festivus— you name it, we want to see it.      All photos tagged with #DeckTheLab...

... courses. The Intro to the Lab Bench series is meant to be an informative introduction for those who ...

...sweaters, Flannel Fridays, Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout...

...CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions describing...

...where we can find the plasmid. While we cannot guarantee that everyone we reach out to will end up depositing...

... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

...families, eating delicious food, or playing one of the giant games or indoor mini-golf course.    While the main...