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We narrowed to 1,147 results for: Gene

Showing: 921 - 940 of 1147 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Are the shots clear enough for the audience? As Addgene’s Science Video Production Specialist, I’m constantly...March 2020, just four months after I started with Addgene, I was about to have a check-in with my boss Jennifer...Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing social distancing and wearing their...traditional solution was completely out of reach for Addgene.  So, onto research! I needed to design a system... needed to figure out a microphone situation. Generally, I record into an external recorder using a lav...the Lab Design: The lab is the beating heart of Addgene. It’s why we exist, storing plasmids, and distributing... out all of the written and video protocols at Addgene? If you need help in the lab, we have you covered...

  2. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...write for a university publication, improving your general writing skills will help you in your academic publishing...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene  Read David Riglar's FOR Boston 2015 Blog Post...

  3. What's New in CRISPR - December 2019

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR... optimized for Burkholderia, they identified 67 genera of Proteobacteria that this toolkit might be used...TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  4. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...” said Guillaume Bourque, a Professor in Human Genetics at McGill University. “There is a need to pool...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

  5. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...people and society. Future generations of IMP Motivating future generations is key to the success of this...from international financial struggle, our new generations feel thrown, to sink or swim on their own, into...knowledge, experience, and new professional capacity generated during this process result directly in positive... growing demand from universities and future generations of professionals has made possible a significant...School, where she studies age-related macular degeneration. She is also a blogger who writes posts for ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...website, Help Center, or email us at deposit@addgene.org. ...

  7. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

  8. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful. Nothing more ...pMal-T-Avi-His/BirA plasmid now available through Addgene. We have successfully used this plasmid to biotinylate...cell signaling.     Additional Resources on the Addgene Blog Check out other Hot Plasmids Find Additional...Posts on Plasmid Technologies Resources at Addgene.org Learn about Popular Plasmids in the Repository...

  9. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...language is ... The general representation of data is... The figures are ... In general, I think that this...language in the first (and clunky) version of my general comments and adjust them if my opinion changed ...can be deposited in the specific repositories (Addgene, WormBase, Arabidopsis.info). These repositories...PMCID: PMC4528400. Additional resources on the Addgene blog Find blog posts on scientific sharing Get...tips on writing a review article Resources on Addgene.org Learn about depositing plasmids pre-publication...

  10. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ...

  11. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...writing for a general audience. The tool highlights jargon terms or words rarely used in general writing in... defining terms. The phrases in Phrasebank are generic, so they need to be edited to suit your particular...desired formatting style. This tool is great for generating citations for a few papers at a time. Mendeley...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...writing a scientific review article   Write for the Addgene Blog ...

  12. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ... Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection     ...single color controls On a flow cytometer, you generally want your positive cells to read between 104 and...isotype controls, while others don’t. However, the general consensus seems to be that if you are looking for...Harvard Medical School.   More resources on the Addgene blog Introduction to Gating in Flow Cytometry Antibodies...

  13. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...communication and epigenetics. Follow her on twitter @kc_palozola.     Additional Resources on the Addgene Blog ...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

  14. Clear and Accessible Writing

    Type
    Blog Post
    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around...the next twenty years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility... accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and Angela — most... in a poster compared to an online abstract or Addgene blog post.  Here, we are presenting this image ... with the rest of the workshop.  For Figure 1, Addgene chose this alt text: "The left panel is a profile...writing!   Resources Additional resources on the Addgene blog Five Steps to Writing Grant Proposals to Grad...

  15. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment...point, but one minute of labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA...host protein critical to Zika replication. Human genetic disease. RaPID can also be used to test how RNA... answer? The RaPID plasmids are available from Addgene! References Ramanathan, M., Majzoub, K., Rao, ...PMCID: PMC4054858.   Additional Resources on the Addgene Blog Study Protein-DNA Interactions with CUT&RUN...resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids Find CRISPR plasmids...

  16. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...Mess in IHC Resources on Addgene.org  Browse ready-to-use antibodies at Addgene...visualize a Y-shaped protein reminiscent of the Addgene mascot Abi? If so, you are not alone. Full-sized... divide up the different regions, both through genetic and proteolytic approaches. This comes in handy...when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken up, there...j.crmeth.2022.100301  Additional resources on the Addgene blog: Introduction to AntibodiesSingle Chain Fragment...

  17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...being adopted by a wider group of researchers. Generating material to empower others to practice and teach...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

  18. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene  ...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...References and Resources More resources on Addgene’s blog Antibodies 101: Introduction to Immunofluorescence...

  19. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within the... of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits of lab...fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to ...krisshamloo.com     Additional Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab...Track Your Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

  20. Antibodies 101: Conjugation

    Type
    Blog Post
    ... Resources on Addgene.org Unconjugated antibodies at AddgeneAntibody plasmids at Addgene... functionality of the conjugate. For example, Addgene’s antibodies are provided in a buffer with the antimicrobial... Schibli R (2015) Antibody Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies ...10.1038/nmeth.4380  Additional resources on the Addgene blog Antibodies 101: Buffers, Storage, and ConjugatesAntibodies...
Showing: 921 - 940 of 1147 results