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We narrowed to 1,149 results for: Gene

Showing: 921 - 940 of 1149 results

  1. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which...them onto the gel. The figure above summarizes generalized expected results for the three primers previously...Overview of Colony PCR Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC ...about Gibson Assembly Additional Resources on Addgene.org Read about Choosing a Molecular Cloning Technique...

  2. CRISPR Plasmids - C. elegans

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    Collection
    ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does...dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty gRNA Expression...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  3. KLF Research Plasmids

    Type
    Collection
    ...depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages with information...KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we...

  4. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Mammalian ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid Gene/Insert Promoter PI ...PI Publication Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids...Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing Other...fused to FokI nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...little as an hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system ...Cas12a however recognizes a different PAM site and generates 5’ and 3’ staggered ends after dsDNA breaks. The...j.cell.2015.09.038 Additional resources on the Addgene Blog Learn about using toehold switches to detect...multiplex genome editing abilities Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR...

  6. How to Be an Excellent Trainee

    Type
    Blog Post
    ...biology you will study. (You can also check out Addgene’s blog, eBooks, and other educational resources ...everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks your mentor...my dissections go smoother next time?” or more generally “How am I doing? Can you tell me what’s going ...personnel! Happy training! Resources Resources on the Addgene blog Trading Spaces – Visiting Scientist Edition...

  7. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...bioengineered scaffolds with patient stem cells to generate compatible functional livers.” Why are you the...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...

  8. Microbiology Resources

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    Collection
    ...page to look for specific genes or applications that are not listed here. Addgene Microbiology Resources ...of Ralstonia solanacearum species complex (Rssc) gene knockout and complementation constructs - Lahaye...Lab Synthetic Biology BIOFAB plasmid set : E. coli gene expression - Endy Lab CIDAR MoClo Parts Kit : Molecular...Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae - Dueber Lab Yeast ...Resources : Browse plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio...Resources for Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses...

  9. CRISPR Plasmids - Prime Edit

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    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Resources Prime editing is a “search and replace” gene editing method in which a reverse transcriptase ...sequence where it nicks the non-target strand and generates a 3’ flap. The 3’ flap binds to the PBS of the...BFP Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... based on plasmid name, species, gene name, and more. ID Plasmid Gene/Insert Vector Type Backbone Mutations...more. ID Plasmid Gene/Insert Mutations PI AAV Plasmids This table contains a general list of plasmids ... to the reliance of the AAV on certain exogenous gene products that mediate AAV replication. This requirement...without the use of a helper virus. Instead, specific gene products can be provided by helper plasmids (e.g...Addgene AAV transfer plasmids Viral...disease in humans. For these reasons, AAVs are generally contained at lower biosafety levels and elicit...AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can ...

  11. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...Career Options Resources on Addgene.org Learn more About Careers at Addgene ...incorporated example documents. This part has generated a lot of positive feedback. Many of these documents...gardener, and cook.  Additional Resources on the Addgene Blog Academic v. Non-Academic Career Decisions...

  12. University of Florida Serotype Testing Panel for the Eye and Brain

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    Collection
    ...vectors for human gene therapy of glaucoma and their reversed cell entry characteristics. Gene Ther . 2020 ...cells by a mutant adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye...Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. PMID: 26390090... Display Exceptional Characteristics for Retinal Gene Therapy. Mol Ther . Jun 3;28(6):1464-1478. PMID:...: Viral Vectors As part of our Viral Service , Addgene is producing ready-to-use viral preparations from...preparations were produced with the pTR-UF11 plasmid ( Addgene #157970 ). Viral preparations are formulated in...

  13. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...skills guide Resources on Addgene.org Learn more about Careers at Addgene Watch out career videos   ...better slides that more effectively tell a story? Generally, the answer to the second question is yes. As ...outdoor photographer.  Additional Resources on the Addgene Blog Find Tips for getting a faculty position ...

  14. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...Here at Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. ...and tweeting it with the tags #DeckTheLab and @Addgene. We’d love to see how you’re decorating your lab...lucky winner? Happy holidays from all of us at Addgene! We look forward to seeing how you #DeckTheLab!...

  15. Plan Your Experiment

    Type
    Collection
    ...mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have a...want to: Generate complete and permanent loss of gene expression or function (knockout)? Generate a specific...cloning your gRNAs. Select Genetic Element to be Targeted The exact region of the gene you target will depend...Considerations Browse Plasmids Knockout Permanently disrupt gene function; cells will use error-prone non-homologous...Prime Edit Repress or Interfere (CRISPRi) Reduce gene expression without permanently modifying the genome...cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying the genome...Intereference : Target the promoter region of the gene of interest, where the Cas9 protein can block transcription...

  16. CRISPR Pooled gRNA Libraries

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    Collection
    ...ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi...each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells ...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman...Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini ...Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd...

  17. Penn Vector Core Partnership with Addgene

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    Collection
    ... distribution via Addgene. For a full list of Addgene's viral vectors See Addgene's Current AAV inventory... from the Penn Vector Core. Chemogenetics (including DREADDs) Optogenetics Recombinases Calcium Sensors... are now available for distribution at Addgene: Penn ID Addgene ID Name Function PI AV-1-49531P 100040...tdtomato Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda...mCherry.WPRE.SV40 Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl...eYFP.WPRE.hGH Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...EYFP Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl...

  18. Addgene Packaged on Request: Scope of Service

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    Collection
    ...Request: Scope of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and... If Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs QC measures...produced through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL ... to toxicity or other reasons For these cases, Addgene will promptly notify you by email within a few ...expectations communicated by email will supersede the general guarantees above. We will have our expert viral...not yield the titer guaranteed here or by email, Addgene will contact you to offer some options for moving...

  19. Zhang Lab CRISPR Page

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    Collection
    ... information, please visit the Addgene SAM library page . Targeted gene activation using SAM There are...available for mammalian endogenous gene activation using SAM: Addgene plasmids #61422 - #61424 were designed...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically...human codon-optimized, and packages both the SaCas9 gene and its single guide RNA (sgRNA) into one plasmid... and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV...-Cre recombinase and sgRNAs targeting the mouse genes: Kras , p53 , and Lkb1 . This plasmid also contains...

  20. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...molecule (see figure to the right). The excitation generated by these two-photons occurs only at a chosen focal...remain. The use of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create...PubMed PMID: 18431356. Additional Resources on the Addgene Blog Get Tips on Choosing Your Fluorescent Protein...Monitor Protein-Protein Interactions Resources on Addgene.org Check out Our Fluorescent Protein Guide Pages...
Showing: 921 - 940 of 1149 results