We narrowed to 1,018 results for: PLE;

1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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   Blog Post

   ...next science challenge. If you have any feedback, please contact us and let us know how you’re feeling.  ...

2. A Better Way to Get Customer Support: The Help Center

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   Blog Post

   ... time to be waiting on hold, let alone waiting multiple days for a reply to an email. You need answers...

3. Future of Research Conference - Remarkable Opening Session

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   Blog Post

   ...Richard Roberts, CSO of New England Biolabs, gave examples of how you don’t have to be in academia to make...

4. 9 Tips to Achieve Success in Academia

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   Blog Post

   ...multidisciplinary one.” Cepko strongly emphasizes that people should “follow their passions, be happy, and match...

5. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   Blog Post

   ...their demonstrated application offer proof-of-principle that CRISPR-Cas9 can be used to target any sequence...

6. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   Blog Post

   ...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

7. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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   Blog Post

   ...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

8. Bringing Sustainable Practices to the Lab: Recycling

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   Blog Post

   ...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

9. Some Like it Hot: Thermostable GeoCas9

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   Blog Post

   ...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    Blog Post

    ...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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    Blog Post

    ...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

12. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    Blog Post

    ...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

13. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    Blog Post

    ...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...

14. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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    Blog Post

    ...Until recently, there were no completely genetic tools that would allow researchers to label just a fraction...

15. Hot Plasmids - October 2022

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    Blog Post

    ...  Multiple Authors...

16. CASTing Off for New Shores in Human Genome Editing

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    Blog Post

    ...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

17. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post

    ...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

18. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

19. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

20. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post

    ...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...