We narrowed to 1,045 results for: STI

...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...

...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...

...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

... and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik ...homology-independent pathway as it involves the direct ligation of sticky or blunt ends and often introduces small insertions...

... expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved by...depending on the delivery method and cell type in question. CRISPR components can be delivered and expressed...

... Gibson and colleagues at the J. Craig Venter Institute in 2009. Here at Addgene we added this option ...These kits also come with specific protocols, suggestions for ratios of product to insert, and tools for...

...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

... about antibody engineering technologies, the Institute of Protein Innovation (IPI) provides information...development methods. Despite recent advances, there is still always room to improve, and scientists will continue...

...1896) where he explores the concepts of animal testing and modifications. The premise of the novel involves...mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy to introduce point mutations...

...purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ...Dewari et al. 2018), however, if you have any questions, do not hesitate to contact me at Pooran.Dewari...

...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types...they are grouped together because they contain a distinct Cas7-type gene. The exact mechanism of type IV...

...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

...Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses...

...announce the launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll ...

...competition, where you can get all your burning AAV questions answered.  Interested? You can learn more here...

...we were able to address these issues using our existing resources. You can read it, and other case studies...

...During the month of January, we’ll be taking name suggestions using this form or the hashtag #BlugeneVirusFriend...

... the past few years, as well as refreshing the existing content. Access the CRISPR Guide here! Highlights...

... our community, we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the...

...photos. The famous Addgene jacket, home to a questionable collection of memorabilia, even made an appearance...