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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,045 results for: STI

Showing: 921 - 940 of 1045 results

  1. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...relied on Sanger sequencing for QC. We occasionally still use Sanger sequencing to confirm an insert that ...nucleotide that passed through based on these characteristic drops. Figure 3: Overview of Nanopore...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ... tag antibody produced by our partners at the Institute for Protein Innovation (IPI), part of a new collection... more highly autofluorescent tissues like the intestine. Figure 3: Expression of fluorophore-tagged...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Lambda Red recombineering, which uses enzymes domesticated from the Lambda bacteriophage, is arguably the...that have only gone through the single cross over still contain sacB and will not grow on sucrose. One ...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... expression is dependent upon the cell type in question. In plant cells, gRNA expression is achieved by...depending on the delivery method and cell type in question. CRISPR components can be delivered and expressed...

  7. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik ...homology-independent pathway as it involves the direct ligation of sticky or blunt ends and often introduces small insertions...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific... GAL80 under different promoters and create sophisticated patterns of UAS-driven gene expression. To further...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...1896) where he explores the concepts of animal testing and modifications. The premise of the novel involves...mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy to introduce point mutations...

  10. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ... about antibody engineering technologies, the Institute of Protein Innovation (IPI) provides information...development methods. Despite recent advances, there is still always room to improve, and scientists will continue...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...we call CRISPR, by any other name would…probably still edit genomes. Understanding the different types...they are grouped together because they contain a distinct Cas7-type gene. The exact mechanism of type IV...

  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses...

  19. CRISPR Guide Update

    Type
    Blog Post
    ... the past few years, as well as refreshing the existing content. Access the CRISPR Guide here! Highlights...
Showing: 921 - 940 of 1045 results