We narrowed to 955 results for: tes

1. Production of Virus in Insect Versus Mammalian Cells

   Type

   Blog Post

   ...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...

2. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...

3. Antibodies 101: Flow Compensation

   Type

   Blog Post

   ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

4. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is...

5. AAV Vector Quality Control: Going the Extra Mile with NGS

   Type

   Blog Post

   ...Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses. In: Methods...

6. Academic vs. Industry Postdocs

   Type

   Blog Post

   ... companies, such as Novartis, have specific web sites for postdoc listings, so the method of posting varies...

7. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   ...is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times...

8. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited...

9. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   ...about your communication style, your potential teammates want to be assured that you will contribute openness...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type

    Blog Post

    ...effects in human genomes (Doench et al., 2016). To test the library, they conducted genome-wide negative...

11. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

12. CRISPR Antimicrobials

    Type

    Blog Post

    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    ...early research career. I've found that research institutes in different places have very different cultures...

14. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...envelope plasmid you use as the choice of envelope dictates the tropism of the virus.  VSV-G is very common...

15. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    ...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

16. How to Write a Scientific Review Article

    Type

    Blog Post

    ...was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers...

17. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    ...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...

18. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    ...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

19. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    ...too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone...

20. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    ...you want to activate, you should also be ready to test three or four guides directed towards that gene ...