We narrowed to 961 results for: tes

1. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   ...engineered with a reduced number of calcium binding sites per sensor (to decrease unnecessary calcium buffering...

2. Viral Vectors 101: Chemogenetics

   Type

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   ...activation is delayed and takes on the order of minutes. Studies looking at immediate effects of specific...

3. Hot Plasmids and Viral Preps - January 2021

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   ...well-characterized Cas enzymes, which rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement...

4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ... Hirsch, M. L., et al. "Self-complementary AAV mediates gene targeting and enhances endonuclease delivery...

5. Gaining Leadership Skills Volunteering at a Professional Organization

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   ...them “Leadership and Management skills”. The NPA states that Postdoctoral scholars should have the skills...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses. In: Methods...

7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ... systems. Some resources will direct you to the latest information about gene function, while others offer...

8. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...localization and transport between healthy and diseased states can also provide interesting insights into disease...

9. Plasmids 101: Protein Expression

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   ...Production of recombinant proteins in plant root exudates Resources on the Addgene Blog Learn about inducible...

10. Production of Virus in Insect Versus Mammalian Cells

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    ...to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in...

11. Viral Vectors 101: Producing Your rAAV

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    ..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...

12. Antibodies 101: Flow Compensation

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    ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

13. Twenty Years of Addgene Sharing: CRISPR

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    ... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is...

14. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's...

15. Degrading DNA with Cascade-Cas3

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    ...unwinding of dsDNA occur simultaneously, which creates a loop in the target strand that is cut occasionally...

16. Academic vs. Industry Postdocs

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    ... companies, such as Novartis, have specific web sites for postdoc listings, so the method of posting varies...

17. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ...is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times...

18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...effects in human genomes (Doench et al., 2016). To test the library, they conducted genome-wide negative...

19. A Guide to Starting Your Own Journal Club

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    ...scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited...

20. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...about your communication style, your potential teammates want to be assured that you will contribute openness...