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...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is...

...Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses. In: Methods...

... systems. Some resources will direct you to the latest information about gene function, while others offer...

...localization and transport between healthy and diseased states can also provide interesting insights into disease...

...is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times...

...effects in human genomes (Doench et al., 2016). To test the library, they conducted genome-wide negative...

...scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited...

...about your communication style, your potential teammates want to be assured that you will contribute openness...

...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...

...early research career. I've found that research institutes in different places have very different cultures...

...envelope plasmid you use as the choice of envelope dictates the tropism of the virus.  VSV-G is very common...

...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...

...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors...

...was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers...

...too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone...

...you want to activate, you should also be ready to test three or four guides directed towards that gene ...

...vesicular stomatitis virus G protein (VSV-G), which facilitates viral entry into the host cell.  Transfecting...