...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

...lysozyme to reduce basal expression levels; expression vector cannot have p15A origin of replication F- ompT...higher T7 lysozyme expression than pLysS; expression vector cannot have p15A origin of replication F- ompT...codons not normally used in E. coli. Expression vector cannot have p15A origin of replication F- ompT ...alleviates inclusion body formation. Expression vector cannot have p15A origin of replication fhuA2 ...Cis-repression of the E. coli T5 promoter (found on vectors such as pQE or similar), inducible under IPTG (...lac repressor on the pREP4 plasmid). Expression vector cannot have p15A origin of replication F-, Φ80ΔlacM15...

...know is your area code. Senators: To find contact information for your senators all you need to do is select...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...Scott Pruitt says carbon dioxide is not 'primary contributor' to global warming The New York Times: With Trump...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

...signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase, leading...to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups...membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...Activity-based directed evolution of a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7....

...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...negotiations can still go horribly awry when several factors aren’t taken into consideration. 1. Always focus...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

...construct (about 8 in Brainbow-1.0 mice.) The combinatorial effect of multiple, independent recombination...woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene...Addgene also has two Brainbow adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY...of neural circuits, such as connections between motor neurons and the neuromuscular junction. In zebrafish...plasmids References: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc...

...combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, ...-CRISPR plasmids Tagging human transcription factors with GFP Kevin White’s lab recently deposited a... of CRISPR plasmids to tag human transcription factors with GFP. As part of the Encyclopedia of DNA Elements...plasmids will help researchers map transcription factor localization and binding via ChiP-seq. Learn ...Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR tool you...

...Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, ... our visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

... take into account chromatin context and other factors, they are easy to use and can reduce off-targets...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would... (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually not very meaningful...and off-target risks will vary depending on many factors. Hopefully now you have all the resources you need...

...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different...options for going into parasitology but another major factor held me back; I do not have a second language. ...allowed me to try out working in many different sectors and to discover for myself what potential job opportunities... Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional Development ...