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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 818 results for: cell

Showing: 471 - 480 of 818 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...there isn’t a template strand left behind that the cell can use to repair. Figure 3: General overview...complex in nucleotide unwinding steps. Molecular Cell, 70(3), 385-394.e3. https://doi.org/10.1016/j.molcel...formation by a type I-C CRISPR Cascade. Molecular Cell, 83(5), 746-758.e5. https://doi.org/10.1016/j.molcel...broad and unidirectional genome editing in human cells. Nature Communications, 10(1). https://doi.org/10.1038...CRISPR–Cas systems for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https:...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... for EM is not something you find in the average cell and molecular biology lab, and EM is limited in ...ultrastructural relationships that influence everything from cell division to memory, but don’t have access to the...fix (and maybe label) your sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample...properties of the sample itself. For example, cultured cells are typically easier to expand than mouse brain ...2016) Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins..., Mohar B, Eddison M, et al (2022) Visualizing cellular and tissue ultrastructure using Ten-fold Robust... Zwettler FU, Guennec ML, et al (2019) Imaging cellular ultrastructures using expansion microscopy (U-...

  4. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...the next step is to transform competent E. coli cells and select the positive clones. The entry clone ... expression of the human KRAS gene in mammalian cells. STEP 1: Generate an Entry Clone There are a few...ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach >99%. ...digest! Then, you can transform or transfect the cells that you want to use for your experiments and verify...Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources on the Addgene Blog...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... attack the cell wall of bacterial cells; however, Mycoplasma species do not possess cell walls. As a ...bacteriophage (viruses that only infect bacterial cells), or a bacterium picking up resistance genes from...conserved, non-negotiable process within the bacterial cell, antibiotic resistance is very likely to develop...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...mammalian cells. This was the first codon optimized membrane protein expressed in mammalian cells. In this...sequence. That means there are 61 possible tRNAs. Cells however, may not express all 61 of these tRNAs and... are also optimized for expression in mammalian cells. For example, Henry Lester’s lab optimized the C...Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels. Journal...

  7. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...your assay will scale. Or you don’t have enough cells to execute genome-wide perturbations. So, while ...lentiGuide expressing only an gRNA, for use with cells that already have Cas9). Each library consists of...be useful when screening for phenotypes, such as cell-non-autonomous effects, that do not lend themselves...

  8. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... nerve and muscle function, hormone release, and cell signaling throughout the body. Because calcium levels...used simultaneously to monitor or control different cell types or signals in the brain. For this to work,... researchers can image multiple brain regions or cell types at once using existing indicators. For example...available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo imaging...

  9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...interaction networks of specific proteins in living cells. Until recently, the enzymes most frequently used...but requires H2O2, which can be toxic in living cells. BioID was derived from biotin ligase and uses far...Alexander Green lab found a solution: a low-cost, cell-free detection assay that can be completed without...family, known to be involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ...

  10. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies... R, Schmalhorst PS, Jude JG, Zuber J, Gerlich DW Cell. 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent... Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role..., Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions...
Showing: 471 - 480 of 818 results