We narrowed to 997 results for: lars;

...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...discovered - many from viruses, but also some from cellular mRNAs. What they all have in common is the ability...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... are short (about 20 amino acids) and produce equimolar levels of mulitple genes from the same mRNA. The...posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information. ...

...actions to a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler...specifically aimed at determining the best options for a particular cell or tissue type (such as here, here, or here...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI 10.1007/978-1-4939-0733... S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume 14. 2017...

...other antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...all bacteria used in research, it isn’t a widely popular selection agent. Pros: Stable (spectinomycin). .../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating...

...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from...V-K CRISPR-transposon systems function in a very similar manner to Type I-F, but instead employ a Cas12k...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

...and injection methods are best suited to their particular scientific question - even when others have already...to successfully replicate published works. Of particular importance to these efforts is the open sharing...Reports are reviewed by Addgene scientists for clarity and accuracy, and by the end of the year, those...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18...occurrence during vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016...

...enhancer it targets is a “pillar of function” for our stem cells. Remove this pillar and the ESC dies. Order...knowledge of which stretches of the genome are pillars for stem cells, or metastasized tumor cells, or... a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional globule of ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

...GC-content. After sequencing and searching for cellular RNAs important for Cpf1 function, they found that...found two that display robust cleavage activity similar to that of Cas9. These two nucleases, AsCpf1 and...respectively), both cleave in a staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9.... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which...et al. suggest that AsCpf1’s off-target rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9...

... and DNA repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...adenine deaminase TadA.   After seven rounds of molecular evolution, they obtained four adenine base editors...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...10.1038/s41587-020-0491-6 Grünewald, J., Zhou, R., Lareau, C. A., Garcia, S. P., Iyer, S., Miller, B. R.,...T. P., Raguram, A., Banskota, S., Newby, G. A., Tolar, J., Osborn, M. J., & Liu, D. R. (2023). Evolution...

...their communication with gene promoters. Cellular and Molecular Life Sciences, 78(19–20), 6453–6485. https...systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX...investigator at the Allen Institute in the department of Molecular Genetics. References and Resources References...Zhou, T., Mortrud, M., Yao, S., Siverts, L. A., Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., ...

..., which is not well protected by Cas9. This is similar to the proposed mechanism for engineered pegRNAs...related voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure...reconstitution via mRNA trans-splicing. By splitting a large gene into smaller fragments, delivering them by ...prime editing, and delivery of disease-relevant large proteins. They demonstrated REVeRT in various contexts...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into...