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We narrowed to 971 results for: lars;

Showing: 471 - 480 of 971 results

  1. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...hard work and talent for many years (at a very low salary I might add). You have a right and responsibility...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep... and learn. Graduate students and postdocs in particular must care about this. The training you get now...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

  2. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...plasmid molar ratio you would like to use for your AAV packaging transfection. In my lab we regularly use ...requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do... Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID: 18265393. ...

  3. Editor's Choice, July 2016

    Type
    Blog Post
    ...Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

  4. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions between crops like...around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning...plasmids he acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase...heavy focus on teaching. He hopes to teach his molecular biology students about the functions of these ...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...indicates that the amount of leaky expression can largely be attributed to the level of spontaneous recombination...) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important...tested in the researcher's system before beginning large scale experiments. This will help to ensure the ...10.1371/journal.pone.0076310 Burger C, Gorbatyuk OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ...Different Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j....

  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery rate... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...positives/negatives, but they all generally yield a similar result and are appropriate to use if all sample...

  7. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ... Read the molecular biology reference Find plasmids for your research Brush up on molecular biology protocols...as pure as for other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are ...consequence, the preparations for this technique must be larger scale, to allow the extraction of enough plasmid...

  8. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...Toolbox: Free Online Molecular Biology Tools by Beth Kenkel There's a lot of free molecular biology tools out...post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins...generate color! They've been adapted for many molecular biology methods such as allelic exchange, FRET...

  9. Let There Be LITE Plasmids

    Type
    Blog Post
    ...limited to particular model organisms or cell types. “This tool is really flexible and modular,” Konermann... vitro by a factor of 10 to 20. A flexible and modular optogenetics tool The researchers also showed just...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...

  10. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...food on special occasions. However, employees regularly bring in baked goods and we have a few unique ...During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...
Showing: 471 - 480 of 971 results