Antibody Validation Using the Indirect ELISA Method
Type
Protocol
...software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated isotype-specific...Collection Antibody Blog Posts Antibodies 101: ELISA Antibodies 101: The Four ELISAs and When to Use Them...Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide Introduction This protocol...Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody incubation Day 4: Secondary antibody incubation...primary antibody and an isotype control antibody to the desired concentrations in the antibody dilution... with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary antibody for 2 ... a purified antigen to demonstrate the expected antibody dose response. Protocols...