We narrowed to 971 results for: Abo;

1. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

2. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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   ...Microbiology, National Emerging Infectious Disease Laboratories.  She is interested in genome replication and...

3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...University of Tokyo, Osaka University). Can you speak about your different experiences in research labs. What...

4. New CRISPR Tools: Cas7-11 and PASTE

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   .... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

5. Viral Vectors 101: Pseudotyping

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   ... also toxic at high concentrations as described above and is inactivated by the complement system in serum...

6. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

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   ...Collection and the BRAIN Armamentarium Collection, a collaborative effort that the Allen Institute contributes ...

7. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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   ...precise and accessible genome engineering. More elaborate protocols are available in our earlier publications...

8. QC Sequencing Technologies at Addgene

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   ...sequencing was considered the gold standard until about 2008 when a new, higher-throughput sequencing-by-synthesis...

9. Antibodies 101: The Four ELISAs and When to Use Them

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   ...conjugate antibodies themselves, the process can be laborious and expensive. In some cases, conjugation methods...

10. Hot Plasmids - November 2023

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    ...section of the Addgene antibody page, and learn more about the whole-brain reference atlas efforts, including...

11. Viral Vectors 101: Preparing Pooled Libraries

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    ...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

12. AAV Titers: Where Do They Come From And What Do They Mean?

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    ... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

13. Plasmids 101: Broad Host Range Plasmids

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    ...plasmids may have high copy numbers and reduced metabolic load (Meyer 2009). Figure 1: Idealized ...

14. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...you enjoy playing with our genes. Have fun!    About the authors: Marjorie Buss is a PhD student studying...

15. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

16. Plasmids 101: Expressing shRNAs for RNA Interference

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    ... & Corey, D. R. (2021). Reexamining assumptions about miRNA-guided gene silencing. Nucleic Acids Research...

17. Hot Plasmids: Summer 2024

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    ...transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration with the Vallabh/Minikel Lab, the Weissman Lab...

18. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    ...limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...

19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ... Addgene. In addition to the plasmids described above, Addgene carries several useful CRISPR toolkits ...

20. Site Directed Mutagenesis by PCR

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    ... Brush up on Your Cloning Techniques Learn More about Plasmids in Our Plasmids 101 Series Use CRISPR to...