We narrowed to 982 results for: IGH@
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TypeBlog Post...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...
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Transferable Skills Guide: Public Speaking
TypeBlog Post...is important to know that the student presenting right before me had been assigned a structure paper with... -
Summer SciComm: Preprints
TypeBlog Post...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about... -
Plasmids 101: Cre-lox
TypeBlog Post...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than... -
Working with Nuclear Receptors
TypeBlog Post...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ... -
Lentivirus ddPCR Titration
TypeProtocol...viral particles. Users may need to run lower or higher dilutions depending on their particular sample....tissue culture treated dish, VWR, 29442-042 DMEM, high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated... Reagent Preparation DMEM Complete: 500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium...increases by a factor of ~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...education. If you suspect that science communication might be for you, don’t wait. Start communicating now.... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ... -
Engaging with science and society at pgEd
TypeBlog Post...for a lay readership, and working with middle and high school students. One of these organizations was ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... job appealed to us. However, since the lab was highly focused on protecting intellectual property, it... -
DNA Quantification
TypeProtocol...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “Highly efficient CRISPR mutagenesis by microhomology-mediated... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...requestors use the plasmid in their own labs. We highly encourage depositors to provide plasmid maps whenever... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...constructs, ADAR2DD(E488Q/T375G) (REPAIRv2) had the highest on-target efficiency and the lowest amount of off-target... -
An Introduction to Adenovirus
TypeBlog Post...vectors all have a genomic capacity that is much higher than other viral vectors (tens of kilobases; compared... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...with one or more of these parameters can lead to a high number of false negatives in Y2H screens. Testing...