We narrowed to 979 results for: LARS

1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   [email protected]. These deposits are considered large deposits and are exceptions to our standard workflow...

2. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   ...raised in the same environment and will harbor similar epigenetic modifications. Special breeding cases...

3. 6 Tips for Grant Writing

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   Blog Post

   ...helpful in assisting me with applications for much larger fellowships. Also, if you were awarded money to...

4. PhD Applications After COVID

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   Blog Post

   ... that provides a new perspective that helps to clarify the harder parts of the application process and...

5. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...guides, which might be rapidly degraded by the cellular machinery, leaving a small amount of active NgAgo...

6. CRISPR 101: RNA Editing with Cas13

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   Blog Post

   ...using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral vectors, they...

7. Tips for arabidopsis transformation

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   Blog Post

   ...seed growth on antibiotic-containing media) A similar process occurs in nature; Agrobacterium tDNA insertion...

8. The Importance of a Fun Workplace: Company Culture at Addgene

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   Blog Post

   ... you are planning, dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary...

9. How to Be an Excellent Trainee

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   Blog Post

   ...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

10. Transferable Skills Guide: Creativity

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    Blog Post

    ... of encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This...

11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org...

12. Lab to Office Culture Shock

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    Blog Post

    ...cross-team projects at Addgene, I find that my vocabulary improves on a weekly basis. There will be meetings...

13. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ...based repressors are useful when knocking out a particular gene isn’t feasible. For example, temporary repression...

15. Weighing Reagents Protocol

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    Protocol

    ...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...

16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post

    ...created in an effort to increase translation in a particular plant species or cell type (Belhaj et al., 2013...

17. Fluorescence Titering Assay

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    Protocol

    ...cells for mycoplasma (Link opens in a new window) regularly Do not over- or under-grow your cells. Thaw a ...

18. Lab Safety for Biosafety Levels One and Two

    Type

    Protocol

    ...immediately, and decontaminate as necessary. For large spills that cannot be easily cleaned, evacuate and...

19. Immunocytochemistry

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    Protocol

    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...

20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post

    ...models remain more difficult to make. For example, large insertions, like a fluorescent protein knock-in,...