We narrowed to 1,010 results for: PLE

1. FlipGFP, a novel fluorescence protease reporter to study apoptosis

   Type

   Blog Post

   ...multicolor reporters that will enable imaging of multiple proteases at once (Zhang et al., 2019). Studying...

2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

   Type

   Blog Post

   ...robot that will “swim” in the river, take water samples at specific locations, measure fluorescent signals...

3. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

   ...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

4. New and Upcoming Viral Vectors - May 2020

   Type

   Blog Post

   ... particular catalog item is available for order please email [email protected] with the vector name and...

5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ... do this but most are labor-intensive, require multiple fluorescent proteins, and aren’t that efficient...

6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   ...ever like to write about a recent plasmid deposit please sign up here.      Listen to this episode of the...

7. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

   Type

   Blog Post

   ...Paratore onto the Board of Directors brings complementary experience and insight to the now 10 member ...

8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   ...state by inducing immunosuppressive measures, for example the upregulation of the immunosuppressive cell ...

9. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   ...peer review that occurs post-publication and is completely open; the reviewer’s name and affiliations, and...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    ...electric current of ion channels in living cells. Multiple properties of a channelrhodopsin, such as cell...

11. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative...

12. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    ..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription...

13. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting...

14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    ...-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse...

15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    ...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...

16. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    ...Lentiviral barcoding libraries have been used for complex applications like lineage tracing in heterogeneous...

17. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    ...plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system, histidine-auxotrophic...

18. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...characteristics that make them more useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about...

20. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ... happens to an organism when a gene/protein is depleted in a knockout experiment. Certain scientific communities...