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  2. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...

  3. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...

  4. How to Lead a Great Meeting

    Type
    Blog Post
    ... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... the following, which are later printed for my physical lab notebook, along with any figures generated.... She is particularly interested in science communication and epigenetics. Follow her on twitter @kc_palozola...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...plasmid. You should also verify the strain and the efficacy of your competent cells (when they get old, they...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

  9. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...Also, it remains challenging to screen for the genetically modified strains that contain your newly tagged...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

  13. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...Preger et al., 2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289...expression selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV ...

  14. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... to deaminate cytidine and used dRanCas13b (catalytically inactive Cas13) to target the cytosine deaminase...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...stretches of sequence could be used. However, a practical limitation is sequencing depth and cost. You need...

  16. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the open conformation (Cofsky et al., 2022), indicating that there is more to learn about this process...conformational state of the HNH domain also allosterically controls RuvC activity through a hinge region...
Showing: 941 - 960 of 1074 results