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We narrowed to 961 results for: ARC

Showing: 481 - 490 of 961 results

  1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...with any scientific research, choice of a technique often comes down to the researcher’s familiarity with...the nature of the experiment, it is up to the researcher to determine which method is most appropriate...stability of the Cas9 protein in the host. Indeed, research has shown that a Cas9 protein from Geobacillus...

  2. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...data of their users readily accessible via web searches; likewise, most in-person events and venues will...understanding of their audience demographics. Do your research so you can go to where your audience already is...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...since we live in the digital age, make sure to research any software you may need: cost or freeware alternatives...

  3. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... doors for scientific research - how will you use these systems in your research? Cary Valley contributed...developed and potential ways you can use it in your research. RNA editing advantages RNA editing has multiple... forward to the expanded use of RNA editing in research and its potential therapeutic uses We are also...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...understanding the brain’s function is knowing its architecture, in particular the connections between different...to their advantageous characteristics for brain research and their ability to introduce genetic material...fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different AAV vectors...Atlas website. A similar approach was used by researchers from the Janelia Campus for their MouseLight ...University of California, Berkeley, the Janelia Research Campus also developed a new AAV variant, rAAV2...

  5. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...proteins included in different CGBE tools allow the researcher to direct the repair of the abasic site. But ... originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...transversion editors were developed, though, researchers at HuidaGene Therapeutics guessed that fusing...existing CGBEs. This distinct approach means researchers can now select the best tool for their application...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...in neuronal circuits or synaptic remodeling, researchers combine the Cre and FLP recombinases to control...combination of Cre, FLP, and inducible systems gives a researcher exquisite control over expression of multiple...Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how...-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...it’s time to use them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing...RNA-guided endonucleases and nickases." Genome research 24.1 (2014): 132-141. PubMed PMID: 24253446. PubMed...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Catharanthus roseus (Figure 1). The ultimate goal of our research is to enhance their production either in the plant...help in screening candidate genes and speeding up research. Certainly, there is still more to transient leaf...your lab, it can become a strong tool for your research. Feel free to add further tips and experiences... in the Lee-Parsons Lab.     References D'Aoust, Marc-André, et al. "Transient expression of antibodies...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...signal at a point on the axis determined by the researcher, based on their controls. You can usually tell...intimated if you’re struggling to understand how the researchers decided to place their gates on the plots! Flow...gating strategy, you can start to ask why the researchers made the decisions they did in their experiments...vitro chondrocytic differentiation: A stem cell research initiative at the King Abdulaziz University, Kingdom...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries. Most of the pooled... pooled libraries and one of our most popular barcoding libraries as pre-made viral preps. If you can’...polyethyenimine transfection protocol. I also recommend searching PubMed for any publications associated with the...feel more confident incorporating them into your research. And, of course, if you have any questions, don...
Showing: 481 - 490 of 961 results