We narrowed to 1,035 results for: URE

1. Mentoring for Scientists: I Have a Mentor, What Now?

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   Blog Post

   ...Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after I get my PhD) and break...

2. Visualizing Translation at the Single Molecule Level

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   ...of the reporter mRNA is labeled by PCP-mCherry (Figure 2). The 3’ UTR also contains a CAAX sequence to...

3. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   ...generating your own fly lines. In the meantime, be sure to browse the publication references and web links...

4. New and Upcoming Viral Vectors - September 2019

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   ... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

5. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   ...fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time...

6. Summer SciComm Series: A PhD in Science Communication

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   ...and numeracy on perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547...

7. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...produced 92% and 87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ... time to choose your own protein purification adventure. You want to purify your favorite protein (YFP...

9. 15 Years of Addgene: The Top 15 Plasmids

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   ...plasmid sharing has certainly taken us on many adventures...From moving office locations three times, to...

10. New and Upcoming Viral Vectors - May 2020

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    ... brain, and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent...

11. Viral Vectors 101: The AAV Retrograde Serotype

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    ...connections, in a powerful way. Using brain structure to understand brain function So far scientists...

12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...cell, this system is clearly also robust enough to ensure high levels of gRNA cleavage.   Xie et. al constructed...

13. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...and train the participants in skills useful for future mentoring relationships.   If you would like to...

14. Summer SciComm Series: Cognitive Load

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    ... understand what you’re communicating. While I’m sure that there are loads of ways psychologists define...

15. The time and cost required to make a plasmid

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    ...much time does it take to make a plasmid? Figure 2: Survey results from a Twitter poll run in 2023...

16. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post

    ... of protein of interest the membrane.   Figure 1: Electron microscope images showing ultrastructural...

17. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ...then digested with the Type IIS enzyme, and the mixture is ligated following a heat inactivation step. ...

18. Plasmids 101: Cre-lox

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    Blog Post

    ... transcriptional and translational termination features with loxP sites on either side (often called a...

19. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...plasmids as well as transfer plasmids. This concept ensures safe handling and expression of these vectors when...

20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    Blog Post

    ...cells in order to determine the specific molecular features that allow such “chemical rescue” in vivo. Engineering...