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...2 mL screw top tube or cryovial and gently mix. Notes: Make the 50% glycerol solution by diluting 100%...

... Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...

...lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to find one ...

... in a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste...

...PES filter or centrifugation at >500 x g for 5 minutes. Do not use the medium if the cells are overly ...