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We narrowed to 653 results for: EPO

Showing: 41 - 50 of 653 results

  1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...factors, the El-Samad lab has deposited plasmids encoding fluorescent reporters and other proteins controlled...random variation, or noise, in reporter expression. To do so, one places reporter expression under the control...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to...deliver the target antibody. TRIM-Away reagents and reporters are available from Addgene - we hope they will...

  2. Addgene: The First Twenty Years

    Type
    Blog Post
    ...years to reach our first major repository milestone — 10,000 plasmids deposited and available — and nearly...service would allow people to add a gene to a repository others could request from — Addgene!  Addgene...meant we could grow accessibility alongside our repository.  “I’m sure you hear it all the time, but Addgene...nearly seven before we had 1,000 depositing scientists. Ensuring that people knew how to access Addgene, and...were making it possible for scientists to both deposit and request plasmids, was a major focus of our ...conferences. And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR...Agreement process, which was a major barrier for depositors and requestors alike. We transformed this process...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...conjugated with a reporter or tag that can then be detected.  Typically, the reporter is an enzyme such...dish and incubate it with a reporter conjugated primary antibody. The reporter reacts with a provided substrate...simplicity we will discuss the more common enzyme-based reporter methods. For enzymatic signal detection, users... sandwich and competitive. In a direct ELISA a reporter enzyme conjugated antibody binds to antigen coated...antibody first binds to the antigen and then a reporter enzyme conjugated secondary antibody binds to ...detection antibody can be directly conjugated with a reporter enzyme or a conjugated secondary antibody can ...).  The sample (purple) is preincubated with a reporter enzyme labeled antibody and then added to the ...

  4. Addgene's Website Update

    Type
    Blog Post
    ...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...easy-to-use depositing guide, with links to our most helpful depositing articles. If you’re depositing data,...paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and the search bar will...Data Hub” link in the Deposit bar. Note that if you’re not logged in, the “Deposit Materials” bar will ...log in or create an account.  Figure 2: The new deposit materials bar New menu Most common activities can...information about Addgene, our mission, links to our deposit, request, and educational resources page, and previews...

  5. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ... countries, into our repository, while expanding global access to our repository through our distributor...plasmids, this represents the largest single deposit in our repository and was a three–year project that touched...libraries, created from 14 deposits, that became available through our repository in 2023.  In June, we announced...developed by our depositors, used by our requestors, and supported by our repository. You can enjoy these...166,247 plasmids. We welcomed 18,899 plasmids, deposited from 1,106 labs in 504 organizations from 37 different... launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll continue ...expanding international access to the Addgene repository, partially through work with our distributors...

  6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...editing tools, the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based...ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot...and N177F mutations). Its excitation maximum is reported as 603 nm, while the emission maximum is 675 nm... ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9...

  7. The MTA at Addgene

    Type
    Blog Post
    ...keep up with the number of requests and deposits in our repository. From 2004 to 2023, we facilitated 784,795...When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit agreement...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority of...party (signatory) to the MTAs for plasmids in our repository. Instead, we facilitate the signing of MTAs by...Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty...agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which your...

  8. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Fluorecent Proteins in Nano Lanterns Reporter gene assays A reporter gene, such as luciferase, usually serves... is also the most commonly used bioluminescent reporter. This beetle emits a yellow-green light with a...several studies utilized luciferase as a genetic reporter in plant and mammalian cells. Luciferase assays...available to choose from) is now a common feature in reporter plasmids.  Learn How Luciferase Can Be Used In...transcription within cells, where detection of the reporter protein or its enzymatic activity is measured....expression can be determined by detection of the reporter in a specific assay, which ideally would have .... Production of photons by this bioluminescent reporter occurs slower than fluorescent-based methods, ...

  9. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... registered reports, the gold standard for testing hypotheses Finally, Registered Reports combine all ...Reproducibility Project: Cancer Biology, where reported methods may not include how many tests were conducted...attention on the results instead of the methods. Unreported flexibility and questionable research practices...analysis code, and other materials. The use of reporting guidelines can also help remind us to include ...creating a time-stamped research plan in a public repository before conducting the experiment (note: registrations...important times to measure that growth rate, you report the results of all four of those tests and not ...fifth time has unpredictably high rates, should be reported as a new discovery that deserves confirmation ...

  10. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...Antibody Data Hub. Data reports  Each antibody submission is used to create a data report, which contains experimental...that information in the report as well.    Figure 3: An antibody data report, showing the results of ...methods section for the data report shown in Figure 3.   At the end of the report, you’ll find a results section... the data report shown in Figure 3. The antibody is rated "Pass". Curating the data reports Just like... Addgene antibody collection can look at user-deposited data detailing how an antibody performed in an... page of the Addgene Data Hub.   Antibody data reports can be searched and sorted by gene/target, name...information is then formatted and published as a report with a DOI for citation. If you’re considering ...
Showing: 41 - 50 of 653 results