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  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas... Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is a sucrase...Biotechniques 40.3 (2006): 355-364. PubMed PMID: 16568824. Hmelo, Laura R., et al. "Precision-engineering the Pseudomonas...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy...Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath of Khan (1982) combined...

  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...achieve your intended next career stage and create a timeline for achieving these goals. Planning is often more...not exist to answer the question or it might be extremely difficult for you to get the needed tools and/...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not sequence all DSBs, may miss unpredicted...Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations...

  11. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...the variable heavy chain fragment of heavy chain camelid antibodies (Hcab) (Arbabi-Ghahroudi et al., 2017...collection! References: Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective...

  12. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...through promoter-directed expression alone is extremely limited. Therefore, highly expressing, generic... privately communicated to me as one of these extremely high expressing promoters). Conduct a time course...

  15. Molecular Biology Reference

    Type
    Guide
    ...may cause certain diseases. Sequencing DNA is extremely important when verifying plasmids to ensure each...only sequence short pieces of DNA. This is not extremely useful when trying to sequence an entire plasmid...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020). Directed evolution of adenine base...Schwaegerle, N. D., Peraro, L., Young, L., Lee, S.-J., Ciaramella, G., & Gaudelli, N. M. (2023). Improved cytosine...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...

  18. CRISPR Guide

    Type
    Guide
    ...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., ...

  19. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...even made an appearance during co-founder and CSO Melina Fan's words of welcome! Executive Director Chonnettia...
Showing: 81 - 100 of 271 results