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We narrowed to 616 results for: GAN

Showing: 121 - 150 of 616 results
  1. Finding Your Science Policy Path

    Type
    Blog Post
    ...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...
  2. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post
    ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...topics in their area of research. The society also organises PhD information events and summer internship information...support each other and provide advice. The society organizes numerous recreational and educational events throughout...for their students. Another large goal of the organisation is to encourage iGEM alumni to mentor younger...Bloggers Learn How to Get Involved in a Profession Organization ...
  3. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...mouse, Drosophila, C. elegans, and zebrafish – essentially all of the standard organisms for which extensive... as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein, troponin...whose association is disrupted in the presence of a ligand (they stick together in one state, but not the ... Kendall Morgan...
  4. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...is a big job, but under the leadership of Nevan Krogan at UCSF we’ve brought together a network of scientists... figure out what is going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct...available shortly. Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking ...FedEx envelopes waiting to be shipped out of the Krogan lab at UCSF. Our team members (shout out to ...be sure to follow us on Twitter (QBI_UCSF and KroganLab), Instagram, and Facebook. Find more COVID-19 ...
  5. 25 Work From Home Tips for Scientists

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    Blog Post
    ... bench scientist who began working remotely recently? Most of us at Addgene began working from home last...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...
  6. Plasmids 101: Modular Cloning

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    Blog Post
    ...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...’s because MoClo specifically refers to the organizational schema described above, and not any particular...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...
  7. Common Injection Routes in Mice

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    Blog Post
    ... available in your lab or organization, and approvals from the organization overseeing your work. Good...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional resources on Addgene.org Genome Engineering...
  8. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership...than you thought! When change happens at your organization, when your boss needs someone to step up, when...likely collected some great time management and organizational skills. If you are responsible for working ...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...
  9. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...variety of model organisms. Use the links below to find Gateway plasmids for your organisms of interest: ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning.... A Genetic Switch: Phage (Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources...
  10. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post
    ...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...questions, panelists and participants of the symposium began providing means to answer some of these questions...
  11. Transferable Skills Guide: Teamwork

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    Blog Post
    ...senior year isolating pheromones from various C. elegans mutants by myself, I would have slowly gone crazy...contribute to the Management Team that steers the organization. As an Addgene vet familiar with the expertise...like-minded group of co-workers who want to make our organization as environmentally friendly as possible. We ...make Addgene the most environmentally friendly organization on the planet, in which case whoa! Mission accomplished...Management Skills Volunteering at a Professional Organization Resources on Addgene.org Check out Career'...
  12. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful!  I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption...
  13. Why Do I Need an MTA Anyway?

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    Blog Post
    ... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even...
  14. Let There Be LITE Plasmids

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    Blog Post
    ...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply... Kendall Morgan...
  15. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post
    ...research is vital in combating misinformation and propaganda that limits access to full reproductive health...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere...
  16. Science as Art

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    Blog Post
    ... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...
  17. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post
    ... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...
  18. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    Blog Post
    ...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...
  19. Viral Vectors 101: Transductions

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    Blog Post
    ...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific...
  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post
    ...tool to advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to...
  21. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...succeed at the same time. I began approaching science funding organizations to request support for our ..., cures, and diagnostics. Almost immediately we began to receive urgent requests for access to coronavirus-related...were facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to ...
  22. Meet the New Addgene Board Members!

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    Blog Post
    ...,” Baradaran says.  Baradaran’s path to science began in high school. “I had a great chemistry teacher...this is what I wanted to do,” Stegmeier says He began this journey in biochemistry made the foray into... at Addgene. While deposits from for profit organizations are less common, they can be very impactful.... diseases and over the past few months, the lab began a project to develop an AAV-based COVID-19 vaccine...
  23. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post
    ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article   ...
  24. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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    Blog Post
    ...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...
  25. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post
    ...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...excitable cells of the nematode Caenorhabditis elegans. They have found several voltage biosensors and...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...may be due to different lipid compositions of C. elegans membranes, which might beneficially influence the...
  26. Antibodies 101: Monoclonal Antibodies

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    Blog Post
    ..., if you are studying a protein that may bind a ligand during your experiment, then you’d want to make...antibody that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody...part of your protein that may be masked while the ligand is bound, then you’ll get a false negative signal...Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological ...
  27. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ...showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...
  28. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post
    ... of informatics. I am a molecular biologist who began coding for my thesis project a few years ago when...language favored by biologists. Almost as soon as I began, I realized that I needed a system to keep track... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...
  29. Will You Be My Mentor? Finding and Asking for Mentoring Support

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    Blog Post
    ...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...
  30. Click Biology: How to Click and Play to Explore Biology

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    Blog Post
    ... Exchangeable HaloTag Ligands HaloTag Reversible binding of fluorescent ligands to HaloTag PCV-Cas9,... acid bases already present throughout living organisms, Click Biology reactions can add new functionality...Addgene in multiple formats: for use in different organisms, targeted to particular compartments, or fused...
Showing: 121 - 150 of 616 results