Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 753 results for: ELP

Showing: 161 - 200 of 753 results

  2. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...member of the Addgene advisory board where he will help shape Addgene's future. Listen to this episode for... Wyss Institute. 33:09 - end : How Alex hopes to help Addgene advance - plants for trimmed down protocols...from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career Path Resources on Addgene.org...

  3. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...new Addgene blog! Same URL, same great articles to help you with your experiments, but a new look. We started... Each topic category contains a overview page to help you get aquatinted. Here, you’ll see a summary of...topic you’d like to see, protocol tips that would help scientists, or a scientist we should interview. ...

  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...combination of interest (Figure 1). It can therefore be helpful to try all three when looking to label an intracellular...can be time-consuming and expensive, it may be helpful to find an antibody that has already been validated...labeling them. We find that cell tracker dyes are helpful in designing such panels, so that pre-stained cell...information on tested antibodies against the target. Helpfully, this also includes clone names, which can allow...

  5. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...Antibody Data Hub Addgene’s Antibody Data Hub can help with this decision. In the open-access Data Hub,...requesting an Addgene antibody, the Data Hub can help you decide if an antibody is a good candidate for...love all data, including negative data, which can help researchers save time and money when selecting an...

  6. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    ...venture capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from...on the Addgene Advisory Board and how he hopes to help young biotech entrepreneurs.   We'd like to thank...to watch both Addgene and Sample 6 grow with his help! Special thanks to our sound engineer/producer, ...

  7. Addgene's Website Update

    Type
    Blog Post
    ...easy-to-use depositing guide, with links to our most helpful depositing articles. If you’re depositing data,...the new search bar, but the menu just above it can help you quickly and easily navigate to educational resources...tools, collections in our catalog, and the Addgene Help Center. A big part of this redesign was pulling ...

  8. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...pandemic. As we adjust, we are reflecting on how to help scientists in this rapidly changing environment.... to understand our role in opening up science to help create new knowledge and research opportunities....both the potential and the promise of science to help a great number of people. I look forward to working...

  9. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    ... uncut DNA and the proper ladder. These controls help you properly visualize your results.The most important...sequencing reaction should be repeated. Guidelines to help with DNA sequencing troubleshooting and analysis... to edit the sequence.   I hope these tips will help you get the most out of your DNA sequencing results...

  10. Science Communication for Everyone

    Type
    Blog Post
    ...collaborate with scientists from around the world and help them share their lab experiences, findings, and ...non-scientist readers about how our articles have helped them understand different topics of science, especially...powerful tool. Use hashtags effectively. This would help you direct your content to the right audience. Avoid...

  11. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type
    Blog Post
    ...committee creates a compensation philosophy that helps guide employee compensation and also serves as an... research.  How will your prior work experiences help you as an Addgene board member? It’s not like I ... dog rescue organization where I foster dogs and help screen dog adoption applications. My most recent...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...overview of reprogramming methods with the goal of helping readers choose a strategy suited to their research...plasmid containing the transposon, along with a helper plasmid that expresses the transposase. The donor...delivered to cells by fusing them with peptides that help mediate their transduction, such as the polyarginine...but there should be at least one method that can help you with your research goals. Are you a new or experienced...

  14. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...and available of in the supplement of this paper), help each person organize his or her thoughts to facilitate...an assessment or critique. When trainees ask for help with various issues, the PI is responsible for determining...’s coming next. Goal setting and career planning help alleviate the anxiety of graduate and postdoc training...

  15. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...Anniversary Gala celebrated all of the people who have helped Addgene grow and spread our mission.  Boston Museum...years ago, Melina Fan had an idea and her family helped bring it to fruition. The concept, though relatively...post was no exception - the rest of the lab team helped to edit and add to this post. Interested in learning...

  16. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...fields with different technical knowledge that could help fight COVID-19. They’ve also collated a list of ...COVID-19 researchers. So far, we have been able to help 65 researchers and we are processing 159 more requests...that we can advance COVID-19 research to hopefully help guide policies and ultimately treatments in a timely...

  17. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...provided with a detailed training schedule that helped me transition easily. Training and responsibilities...independent work on the QC team. I was responsible for helping other scientists on the team ensure the deposits...time, there were no issues. Another example of helpful flexibility was the ability to work either remotely...

  18. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...as the reference for its original publication. To help facilitate this process, we explain how to cite ...this? Not only does citing plasmids from Addgene help others find them later if they need to use them ... collections pages, please shoot us an e-mail at help@addgene.org. We’d love to hear your feedback. Additional...

  19. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...able to do something that was challenging while helping us share our resources better with the community...discussion and questions. I achieved my goal of helping everyone understand the importance of using analytics...else on the marketing and content teams for their help and support throughout the past six months!...

  21. Advice for Choosing a Research Project

    Type
    Blog Post
    ...some important factors to consider and summarize helpful advice from the successful PIs who were previously...assistance from people who have the required expertise to help move your project forward? Most projects typically...selective, and talking to peers and mentors will help elucidate which project is right for you.   Acknowledgments...

  22. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...identified a novel gene, Mettl7a1, whose expression helped coax cells towards successful iEP reprogramming... generating iPSCs Learn how fluorescent proteins help researchers study single cell dynamics in model ...

  23. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... structural and packaging genes, the adenoviral helper plasmid which contains the proteins needed for ...can be organism or tissue type-specific, which can help you restrict the expression of your transgene to...transcribed which aids in nuclear export of mRNA and helps increase the expression of your transgene. The WPRE...

  24. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...secondary, then the following three strategies might help!     Figure 1: Species on species staining...tissue processing and sample preparation can also help reduce the levels of endogenous IgGs in your samples...non-specific staining! Hopefully, these solutions will help get your experiments back on track.  One final note...

  25. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...plasmid. One of the most frequent problems that I help troubleshoot involves viral vectors and plasmids...incubation temperature to grow bacteria more slowly helps reduce the risk of recombination. Look for the “...Tips for Troubleshooting Viral Transductions will help with the next steps.   References and resources...

  26. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...some of the big problems that optogenetic tools can help us to address? Boyden: There are two concrete areas...biggest ones. And as a third example, it would be helpful to have molecules with better two-photon properties... we've hosted hundreds of visitors in our lab to help them understand their specific problem. Every species...

  27. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...engagement. I figured, an engaging card game could help me get the engagement I wanted. I therefore started...your projects with us! This exchange of ideas will help improve life science and biomedical education for...teaching developmental biology Learn how BioBuilder can help teach students about Synthetic Biology Find Arabidopsis...

  28. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...working with cell culture and who was extremely helpful since she was familiar with library generation.... learn from this workshop? These questions will help me avoid being overwhelmed by all the new information...same if you ever go to a workshop because it will help you think about the most useful pieces of information...

  30. The MTA at Addgene

    Type
    Blog Post
    ...changed a lot over the last twenty years! This has helped us develop a fast, agile approach, which we’ve ...process, you can check out our Tech Transfer Webpage, help center articles, and our MTA YouTube video for more...Coughlin, Darcy Morse, and Matt Takvorian for their help with this post.   ...

  31. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... academic research community. At Addgene, we’ve helped distribute many of these technologies in plasmid...target cells, and, finally, conduct the screen. To help alleviate some of this labor for our users, we now...services. Please send any questions or comments to help@addgene.org. Finally we’d like to thank all those...

  32. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... are fun, but getting buy-in when delegating can help ensure successful results.  Can you provide the...minutes and plan changes. Written documentation helps make sure everyone is on the same page. Effective... Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete Goals ...

  33. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...experiments. This helped reinforce our goals of making more AAVs and educational content to help researchers... to us, request that we start making the AAV, or help us produce new educational content on the blog. ...

  34. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...cold temperature locales and psychrophilic enzymes help them survive under severely cold conditions. To ...any food processing industry as it can potentially help minimize the incidental losses like spoilage and... and the myriad facilities offered by them which help foster a more productive and collaborative environment...

  35. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...application, our Viruses as Biological Tools blog can help you learn about the different types of viruses and...  Clinical applications Viruses in vaccines helped pave the way for their use in other clinical applications...could further your efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services...

  36. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...ll flood out other signals. But PE can be very helpful if you're trying to find a relatively rare protein...antibodies can get a bit complicated, but hopefully this helps you successfully select - and keep! - antibodies... January 31, 2022, from https://flowcore.syr.edu/help/spectral-overlap/   More resources on Addgene’...

  37. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...summarizing the conclusions of your paper. This will help the editor handling your manuscript decide on whether...English speakers) to proofread your manuscript and help you correct any language- and spelling mistakes....Arguing will make you look unprofessional and doesn’t help your chances of getting your manuscripts published...

  38. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...breast cancer. Infectious Disease. RaPID can also help identify which host proteins interact with viral...interactions. Do you have a question that RaPID could help answer? The RaPID plasmids are available from Addgene...

  40. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our customers...internal referrals. Our long reaching reputation helps us hire great people from farther away via connections...
Showing: 161 - 200 of 753 results