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We narrowed to 801 results for: MAK

Showing: 161 - 200 of 801 results

  1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...find events stressful. Video chatting makes it pretty easy to make 30 minute appointments with people all...almost 6 months, you may be looking for more ways to make sure you are moving forward. Here are a few out-...hand at a video presentation? Most video platforms make it easy to record yourself. You can watch yourself...informational interviews, for more details on how to make the most of these exploratory conversations. If ...least two informational interviews a month you will make good progress on both building relationships and...

  2. The Strength of Story Telling

    Type
    Blog Post
    ...arsenal to make sure people pay attention. Highlight your lowest points and highest points and make clear ...crucial part of interpersonal communication, which makes honing your ability to tell stories a similarly .... Whatever you’re working on, start wherever it makes sense to start and try to be as chronological as...protagonist In most cases, the protagonist will be you. Make sure to highlight what lessons you’ve learned and...failures. Foster conversation A story is not a lecture. Make yourself open to conversation — put your work on...

  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...and construction steps are complete, it’s time to make virus! The constructed viral vector containing the...particles are secreted into the cellular media, making viral collection fairly easy. Producer cell media...similar outcomes, so why and when should you opt to make a virus instead of just zapping your cells? That...transfect’ cells lines are amenable to transduction, making it a logical choice for efficiency. Where viruses... rarely done, viral pseudotype can be altered to make the virus specific for a type or subtype of cells...with alternative methods. Ready, set, transduce! Making virus can sound daunting, but it is easier than...

  4. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ... are sensitive to blue light. Many have tried to make green molecules, but then alongside blue they actually.... So we developed a red molecule. We also had to make the blue light molecule much, much, much more light-sensitive... optogenetic tools in a deep part of the brain, making it sensitive to light. When they illuminated this... emotions, to see how a monkey changes decision making, to cure a human patient. We can't do it all by... I would say we still would like to find ways to make these tools more selective for different ions. Right...interesting. It would also be great if you could make neural silencers that require less power. Those ...

  5. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...can revisit the Q&A in the chat box. Presenting? Make it interactive! As a presenter, it can be difficult...giving a research talk, there are still other ways to make your session more engaging, for example, by using...to keep your video on and look into the camera to make it seem more like you’re connecting with the people...over. Depending on the conference, organizers may make the poster available after the conference is over...getting to know the app or virtual platform and making sure you stay connected. If possible, test out ...experience as an on-site conference, you really have to make space for it,” says Bachle. “On the flip side, you...

  6. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...components. Making proteins outside of a cell - cell free extracts Typically, if you want to make a protein...more and more molecular biology-based activities making their way into high school classrooms, like gel...cost-effective, as each one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable...they can touch. With BioBits, we want students to make the connection between the facts they’ve learned...experiences and knowledge with each other to help make learning biology more fun – bit by bit. Ally Huang...

  7. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...over-diluting, and make sure to remeasure your titer after diluting to confirm. Luke Makana Hanley is a Research...Check out this post for a refresher on AAV titers Making contact with a cell Although an individual AAV ...see the dwindling proportion of AAV particles that make it through each part of the cell on their way to...additional best practices. Keep your cells happy - Make sure your cells are as healthy as can be to prepare...filter - When using a concentration filter column, make sure to rinse off the surface of the filter membrane...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read any...success prior to your departure for the new placement. Make sure you read any relevant literature for the techniques...you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist... a structured visiting program, you will have to make arrangements on your own. This will include, but...a guest and likely a trainee in your new lab, so make a good impression! Remember that your host is going...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... ability to make discoveries." If you ever run out of ready-to-use virus and want to make new lentiviral...genome editing tool is its developers’ willingness to make their CRISPR technologies available to the academic... reduced titer associated with large inserts can make lentivirus production frustrating - a researcher...protocol only to find that the low titer of her prep makes it unuseable. With the large size of the SpCas9 ...biology you're interested in studying. You need to make sure your assay is scalable and robust. What you...

  10. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...important part of being a “good scientific citizen” and making sure you do what you can to help your fellow researchers...consistent way so other researchers can use them, to making sure that methods and protocols are available and... this communication and sharing fits together to make for a transparent and collaborative research environment...for being transparent in their communication or making their data and reagents readily available. What...confident that everyone’s enthusiasm for actually making it happen would increase dramatically. I’m not ...resources on the Addgene blog Find more ways to make your research more reproducible Read all of our ...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    ...running one, you’ll know there’s a lot of choices to make when designing the experiment. What detection method...    Figure 1: The technical decisions to make when designing a western blot.  Psst! Looking...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...blocking buffer and a recommended concentration. That makes the choice easy! Protein-based blockers include ...Chemiluminescence kits When activating your enzyme, make sure to choose a chemilumescence kit appropriately...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...proteins diffuse semiradially during the transfer, making membranes inherently “fuzzier” than gels. Quantification...

  12. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...variable actually makes a big difference for the robustness of the findings.   How do we make research more... noise in the process of sampling and inference-making. Even perfect transparent and rigorous studies ...with a specific statistical test.  Preregistration makes clear the distinction between hypothesis testing...With Registered Reports, the authors are able to make that clear distinction between hypothesis testing...Furthermore, the journal and reviewers are incentivized to make sure that any possible null is really a true null...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... 2015. What makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for...a point mutation in the puromycin cassette that makes it less efficient in some cell lines. pSpCas9(BB...V2.0 was made available through Addgene in 2015, making this the most recently available plasmid in the...a point mutation in the puromycin cassette that makes it less efficient in some cell lines so it may be... thank all of our depositors and requestors for making their reagents available and for supporting scientific...

  14. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...similar to previously discovered Cas13 enzymes - what makes these orthologs so special? The first property is...Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other Cas13s and a whopping...whopping 33% smaller than Cas9! Their small size makes then easy to package in low-capacity vectors like...broader regulators of Cas13d activity. This property makes WYL proteins an intriguing counterpart to anti-CRISPR... yielded >80% knockdown. It seems that CasRx may make it possible to target essentially any RNA in a cell...and we at Addgene appreciate our depositors for making their tools available to the broader community....

  15. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...cargo is designed to make you sick…well, you feel it. But the same features that make these familiar viruses... and deposit a DNA/RNA cargo into their targets makes them attractive and tractable tools for genome engineering...essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives? Well, for one...Secondly, some viruses have a low immunogenicity which makes them ideal for applications with whole organisms... when introduced into an organism. This feature makes them an attractive tool for human subject delivery...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... aided design) drawings of their devices, which makes it very difficult to adapt the device to one's own...2020) or openUC2 (Diederich et. al., 2020) aim to make microscopy as a tool accessible to practically everyone...growing number of "community-driven" software that make everyday microscopy much easier. On platforms such...sources such as Amazon, Ebay, or Aliexpress, which makes it difficult to develop accessible open-source hardware...Distributing hardware via globally established makerspace networks and training users via workshops, seminars...newly developed techniques are the key enablers for making assemblies open-source. The sharing of digital ...

  17. How Addgene Creates Content

    Type
    Blog Post
    ...we choose topics for the Addgene blog? Or why we make certain videos? In this post, we invite you behind...experiences from thousands of laboratories that makes up our Help Center, blog, website, social media,...process led to quick dives back into the literature to make sure absolutely everything was correct. Final touches...between our content team and our graphic designer to make sure things are clear, accurate, and in line with...shown.   However, for our blog posts, we usually make figures in BioRender or use appropriately licensed...review to catch those pesky typos, and hit publish. Making content is an iterative process. We are constantly...

  18. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...light-gated ion channels such as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory...causes it to fire. Created with BioRender.com. To make channelrhodopsin-based optogenetics minimally-invasive...channelrhodopsins. Their new channelrhodopsins, called ChRgers, make the brain a little easier to access with optogenetics...injection. This lower multiplicity of infection (MOI) makes it challenging for ChR2(H134R) to function because...showed that ChRger2 stronger photocurrent properties make it better suited for systemic delivery with PHP....TL;DR The new channelrhodopsins, called ChRgers, make it easier and less invasive to use optogenetics ...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... unique cutting features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering... into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic delivery by... to give it a go? The high specificity of Cas14 makes it a promising detection tool for rare, often hard...unlike Cas9, it targets RNA instead of DNA. This makes it particularly useful in generating transient edits...target sequence. The RNA-targeting ability of Cas13 makes it perfect for targeting mRNA and useful as a mechanism...targeting abilities of the other Cas family members make for useful implements in the scientist toolbox. ...

  20. Lab Automation at Addgene

    Type
    Blog Post
    ...your way. Label your tubes with a label maker. Label makers generate far more legible labels than your...into the lab wishing there was a machine that could make your life a hundred times easier. We’ve certainly...Addgene recently started automating a few protocols to make standard lab processes easier and more efficient...really liked that Beckman was flexible and willing to make removable parts to fit on the deck of the Biomek...pick up 8 tips and position the tips indepently making it easy to spread out your samples. This can be...

  21. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-based immune systems in...broken down into different types, with Cas3 systems making up type I, the most abundant type in the Class ...away at both the non-target and target strands by making cuts every so often. Cutting both strands ensures... for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s more powerful than it is. The...issues with expressing all Cascade components can make Cas9 easier to work with than Cas3. Future targets...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

  22. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... we’d like to make sure they are present at a very low concentration. So how do we make sure to catch ...an experiment because of poor reagent quality. To make sure our AAV vectors are of the highest quality,... mission of accelerating scientific research. To make sure that adeno-associated viral (AAV) vectors available...robots. Unfortunately, humans have been known to make mistakes. What if a tube is mislabeled or someone...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

  23. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...be “let’s try to make this STEM activity fun,” but rather “let’s see how we can make this fun activity...time is giving fewer examples and think of a way to make this section more interactive. If you can think ...good analogy that involves student participation, make it happen! How should we use CRISPR? How shouldn...students that scientific history was very much in the making. One of the exciting, and challenging, aspects ... Perhaps you have young children and you want to make sure their STEM education is supplemented. A 2009...

  24. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J,...Non-SpCas9's bind a variety of PAM sequences, which makes them useful when no suitable SpCas9 PAM sequence...non-SpCas9's may have other characteristics that make them more useful than SpCas9. For example, Cas9 .... BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM which makes it easier to target AT-rich genomes (Kleinstiver...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...

  25. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...reliable and robust high quality viral vector service, making an array of ready-to-use viral vectors available... prioritization process to decide which tools we make available. This prioritization happens for good...of our most popular ready-to-use viral vectors, making up almost 10% of our requests by 2019 (Figure 5A...which preps to include in our service allows us to make ready-to-use viral preps that are available quickly...Hub before selecting an AAV can help a researcher make a more informed choice on which AAV to request and...since, we’ve learned so much about what it takes to make these viral vectors not only available, but accessible...

  26. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...to be a part of projects that connect people and make you work in a collaborative environment. Think of...conversations about your ideas and don’t be afraid to make something concrete happen. You may have more willing... institutions personally on conference calls and make sure to maintain these relationships by keeping ...organization. You can meet collaborators  for lunch, make a point of talking to them at a seminars, or even...from an internal to an external connection is to make sure you keep open lines of communication with colleagues...see interesting events, articles and newsletters I make sure to share them with a short note - this creates...

  27. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ... Denotes the presence of an additional plasmid-- make sure to maintain this by growing on appropriate ...Induction timing is important, as you typically want to make sure your cells have first reached an appropriate...exponential growth phase are alive and healthy, which makes them ideal for protein expression. If you wait too... as there are not enough cells in the culture to make protein.  The DE3 lysogen/T7 promoter combination...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...Double-check your plasmid backbone and expression strain to make sure they are compatible. An arabinose-inducible...

  28. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... can promise is that my suggestions can help you make the best case for your candidacy. A major reason...complains that a seminar was too easy to understand. Make your seminar accessible. Let the experts ask you...minutes of the chalk talk. Realize that it is rare to make it through the details of all of the aims of a chalk...points you want to convey for each section or aim. Make sure those get priority. The fine details for aficionados...examples are not especially helpful. I set out to make something practical and accessible. If there's something...postdoc salaries all too well. If there's a group to make money from, postdocs are not that group. If you ...

  29. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...even one less run a week makes a big difference. Clean out freezers so you can make room and don’t have to..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...sustainability problem, how can one little change make a difference? Individual changes to our behavior...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...

  30. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... puncta in each cell. When making figures for a paper or presentation, make sure the images are accessible...want to look for them? Or maybe you just want to make some really beautiful images? Multiplex IF may be...different colors in one session – fast and easy! Just make sure your primary antibodies are all of different...considerations to keep in mind. Most importantly, make sure you are using primary antibodies from different... and dye meets your needs and budget, or you can make your own. For example, to label the rat brain sample...

  31. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...requires that the RNA be tagged. “This tool makes it possible to make the protein specific to any endogenous...PUF proteins! A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins to hit...continue to evolve and improve, Abil says they will make those new plasmids available through Addgene, and...the final version,” she said. “If we learn how to make the library even better, we would be very happy ...

  33. Water Bath Protocol

    Type
    Protocol
    ...appropriate water level in the water bath so before use, make sure that there is enough water inside....

  34. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...people to take a break from their everyday tasks and make time for special projects? We borrowed an idea from...great success at accomplishing “we-should-really-make-time-for-that” type projects. Cross-team “Random...communication between labs. Scientists are often willing to make time for free food, and these lunches would provide...between labs, and people work hard at Addgene to make plasmid sharing as easy as possible. However, I ...work to share time with the people around us. It makes the quality of our work better and keeps us smiling...

  35. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... or set of plasmids. We hope that these articles make it easier for you to find and use the plasmids you...for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic animals.   Wall...To expand the capabilities of Gibson assembly and make a more modular platform, the Jacks lab generated...cumbersome to use in a high throughput manner or make it difficult to quantify interaction strengths. ...Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...introduction of auxin range from 10-20 minutes, making AID an appealing tool for studying essential proteins...described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using CRISPR technology...

  36. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and antibacterial ...interpretation. Also, the signals from the nuclear area make the detection of mycoplasmas difficult (Ligasová...contamination when you are working without gloves or masks. Make sure to change the gloves and masks regularly instead...line stock for mycoplasma contamination before use. Make sure there is no contamination present in the serum...disposable pipettes to avoid cross-contamination. Make sure you keep all the required equipment and reagents...Avoid long exposure of the cell culture to air and make sure to tighten the flask cap before placing them...

  37. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...This is something I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate...articles (gasp). To my delight, not only was I able to make the edits without any reagent-grade endangered Martian...Vinculin is a loading control. 3. In terms of making the monoclonal line: I had never done it before...important results (for example, if you needed to make a figure with the data). Using acrylamide instead...agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene knockout was straightforward...who you are, you could quickly be on your way to making new scientific observations. References 1. Rago...

  38. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...distributed. Next month, we are making two changes to the policy that will make the process a bit easier and...citation and release the hold. Increasing impact Making the plasmids available as soon as your paper is...publication isn’t required to share your plasmids! You can make your deposit available before publication and we...Addgene! We'll continue to review our policies to make sure they meet our goals, maintain your trust, and...

  39. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...this responsibility seriously. Every decision we make, every project we undertake and every service we... that our materials are affordable but enough to make us self-sustaining. With this unique model, we are...opposed to fundraising.   Aside from enabling us to make all decisions with the goal of  benefiting our scientific...ever curious what specifically drives the decision making process at Addgene, just look to our core values...curated, high-quality data and reagents Addgene makes it easy for scientists to deposit and request plasmids...

  40. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ... science brick-by-brick”. Our mission is to help make science mainstream and to show people the very human...at these struggles will bring people together and make these daily challenges a little easier to overcome... As we started building, we thought – how can we make the connection between LEGOs and science more concrete...and sounds to synchronize with the images thereby making the videos more real. To give you an idea of the...amount of work, see how many pictures we take to make a walk cycle of one step. Challenges in the LEGO...
Showing: 161 - 200 of 801 results