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  1. Summer Fun at Addgene!

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    Blog Post
    ...behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be ...means summer is right around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've...We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing... be posting in the next few weeks.)  Finally, Addgenies love sunshine and warm weather - check out our...

  2. Countdown to Halloween @Addgene

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    Blog Post
    ...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...celebration this Friday will combine all of these. Addgenies have been organizing Halloween festivities for...

  3. A Look at Addgene's QC Process

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    Blog Post
    ...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...bacterial growth. Created with BioRender.com.   At Addgene, we put a lot of effort into our quality control...sequence, as they were deposited before 2017 when Addgene started sequencing all incoming plasmids. If you...

  4. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post
    ...grants and Addgene’s mission This is the first NIH grant that Addgene has received. Addgene prides itself...allow Addgene to further our mission of scientific sharing,” says Dr. Daniela Bourges, Addgene’s interim...antibodies Resources on Addgene.org Find recombinant antibody plasmids at Addgene ...to improve upon these critical research tools. Addgene is excited to announce that we have recently received...Neurotechnologies® (BRAIN) Initiative, will allow Addgene to create a much needed open-access recombinant...recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access benefits to antibodies...single-chain variable fragment (scFvs) and nanobodies. Addgene will produce a subset of this collection as ready-to-use-proteins...

  5. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post
    ...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...researchers around the globe. Addgene’s educational resources As Addgene built out our scientific support...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel...about virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...accessible, due to a wide range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical...accessible weren’t always the same thing, shaped both Addgene’s founding and our growth into the company we are...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...the model organism, injection site, and titer. Addgene’s Viral Vector Service Besides being complex to ...quality of the prep. This was a large factor in Addgene’s decision to launch our viral vector service in...able to order high quality viral vectors from the Addgene repository can save researchers significant time...

  7. More Data for You: Find Articles Citing Addgene Plasmids

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    Blog Post
    ...ICYMI: Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...Plasmid" list. Email us at help@addgene.org with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ...Exciting news! Addgene recently rolled out a new feature on our plasmid pages - links to articles citing...systems it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications,... see where they've been cited. Benefits to the Addgene community of scientists Benefits to depositing ...Properly citing plasmids you've requested from Addgene may get your paper listed in the "Articles Citing...you know of an article that used a plasmid from Addgene, we want to hear about it so we can link to it ...

  8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. References...

  9. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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    Blog Post
    ...features of Addgene's website Resources on Addgene.org Try out the new search on Addgene's homepage ...been working on behind the scenes here at Addgene.  Addgene's new homepage While users are increasingly...emphasize the values that make Addgene unique: community, sharing, and fun. The Addgene repository is built on...  Additional resources on the Addgene blog Learn more about Addgene's new search tool Read more about...-over and our new homepage? If you've visited Addgene’s homepage recently, you probably noticed some big...access the content they need. All within seconds.  Addgene’s old homepage had been relatively the same for ...shares reagents and knowledge with one another, and Addgene make this process easy and fun every day.  With...

  10. Anatomy of a Plasmid Page at Addgene

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    Blog Post
    ...We Do Quality Control at Addgene Resources on Addgene.org Join the Addgene Community Learn the Benefits...plasmid. Use the Addgene catalog number to easily find template citations for Addgene plasmids you have...information is also available from Addgene’s sequencing reactions (‘Addgene Sequences’).  These sequences ... hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read about Our New Citations... to find this plasmid through search is... The Addgene plasmid number: This is the unique number given...Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication of popularity. A yellow... this PI’s plasmids that are available through Addgene. Publication: If the plasmid has been published...

  11. New Educational Resource: The Addgene Videos Page

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    Blog Post
    ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their.... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

  12. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...hands of our finance team, now 3.5 Addgenies strong. The Addgene Board of Directors is mature and poised... Turnover at Addgene is about half of what is standard in most sectors. The Addgenies support a high... ahead of the curve on this. Addgene’s success is about the Addgenies. I never worry about their dedication...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...

  13. Addgene's 20th Anniversary Party

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    Blog Post
    ...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...travel to Addgene's headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the...the years, including an album of Addgenie pet photos. The famous Addgene jacket, home to a questionable ... each and every Addgenie, from our co-ops to our directors, we can't celebrate Addgene without recognizing...This year marks Addgene's 20th anniversary! We've been celebrating throughout the year, and on Sunday... the party. There are so many teams that make Addgene functional, handling everything from facilities ...  Throughout the party, we shared memories of Addgene's past. Framed photos and slideshows shared some...

  14. Addgene Protocols Go Viral: AAV Purification and Titration

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    Blog Post
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...website Watch Addgene's video collection ...

  15. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

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    Blog Post
    ...recently awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Resources on the Addgene Blog Check Out Posts on Synthetic Biology Learn about the Addgene, Seeding Labs...In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University... research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists... biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's...Sciences in Ghana. He is a recent recipient of the Addgene, Seeding Labs Plasmid grant and is working on a...

  16. 2023 and 2024: Reflections and Looking Ahead

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    Blog Post
    ...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...mirroring our own aspirations for Addgene. On behalf of every Addgenie, thank you for twenty years of incredible...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary. Throughout the ...

  17. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

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    Blog Post
    ...plasmids deposited at Addgene from the abstract page. Check out that beautiful Addgene “Find Plasmids” badge...LinkOut” and search for “Addgene”. Then check the “Link Icon” box next to the “Addgene Nonprofit plasmid repository...Manuscript Resources on Addgene.org Learn How to Order Plasmids from Addgene Check Out Our FAQ Pages ...badge to be taken directly to the plasmids on Addgene’s site (Side note: I always recommend you read the...plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create an account... hope this helps! Additional Resources on the Addgene Blog Get Tips on Keeping Up with the Literature... addgene...

  18. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ...materials from Addgene can qualify. We also reward improvement against one’s own record Mention Addgene Master...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ... for depositor and requestor alike. That’s why Addgene works closely with TTOs to help protect our depositors...

  19. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    Blog Post
    ... we can make Addgene better and make scientists’ lives easier. Why transition to Addgene? So why did ... that PhD. Resources at Addgene Read Other Careers Posts Visit the Addgene Careers Page Learn about ...Harvard University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days... less time wrangling bacteria and instead help Addgene fulfill its mission of helping scientists share...did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the lab like the cartoon...didn’t, it would have been unwise of me to join Addgene. This is particularly true in my position as an... here! Thus far being an Outreach Scientist at Addgene has been awesome. The process of writing, editing...

  20. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ...reading this blog, chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ... green DNA background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background...transformations with the plasmids you receive from Addgene? Our bacteria background is probably a good fit...

  21. Hot Plasmids - August 2020

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    ...pMAK461(NBαRabitb-IgG-Fc, Addgene #140699) pMAK462 (NBαMouse/Rat-IgG1-Fc, Addgene #140700) pMAK463 (NBαMouse-IgK...NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) The pMAK461-64 plasmids were expressed...the plasmids to set up your screening systems at Addgene! Schematic of the evolution platform to generate...fluorescent mammalian cell structure markers plasmids at Addgene! Chertkova, AO, et al., bioRxiv. 2020. https://...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...human lung epithelial cells. Find the plasmids at Addgene The MoClo CRISPR/Cas Toolkit for plants includes...

  22. Learning Technical Skills at a Lab Central Workshop

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    Blog Post
    ...Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...information from the workshop. Future workshops for Addgene Addgene strives to be a great resource for the research...Technician at Addgene who is interested in medicine and music.       Additional Resources on the Addgene Blog ...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ... use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about...excited to go there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext...libraries as opposed to replicating them like we do at Addgene), going to the workshop was a great opportunity...

  23. Why Do I Need an MTA Anyway?

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    ... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... review by the Provider Limited liability Addgene makes it easy Even with all those benefits, we ... handle all the paperwork. Record Keeping: Addgene will let you know exactly how many times your materials...Streamlined Processing: When you place an order, Addgene generates the MTA and emails it straight to you...

  24. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post
    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping...  Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order...Suggest A Plasmid You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which...deposit spreadsheet. The data entry process On the Addgene home page, if you scroll over “Deposit” in the ...

  25. Stepping into a Virtual Internship: My Experience at Addgene

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    Blog Post
    ...their roles at Addgene. I had a great time speaking with Quintin Marcelino who is Addgene’s Science Video...change. Having been at Addgene for a month, I can say with certainty that Addgene is a company that cares... continuing Addgene’s Meet an Addgenie Series on Instagram. I interviewed fellow Addgenies about their...like to work at Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke with amazing...Sociology and Dance at Skidmore College. I was also Addgene’s Marketing Intern this summer! With the help of...made me intrigued by the marketing internship at Addgene. Although my interests are very clear, I had no...COVID was getting, I was hoping to walk into the Addgene office my first day and meet everyone but this ...

  26. Celebrating One Million Plasmids Shared!

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    ...fit in a freezer). Our Addgene celebration was so much fun! Here's the Addgene Leadership team in all ...excitement amongst our Addgenies on social media or around the office. @Addgene congrats for 1 million... a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...might have you wondering how many plasmids leave Addgene headquarters every day. That would be an average...our shipping room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory...it takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on... — BryanRoth (@zenbrainest) August 21, 2018 As Addgene grows, we have been able to adapt to the needs ...

  27. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

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    ...became a member of the Addgene advisory board where he will help shape Addgene's future. Listen to this...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...In this episode of the Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology ...Institute. 33:09 - end : How Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution...us.  Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board with a particular interest...Additional Resources on the Addgene Blog Learn More About Alex's Work with Cas9 Activators Michael Koeris...CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ...

  28. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

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    ...in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis Bengyella Dr. ...would like to use the plasmids he acquires through Addgene to produce molecular biological tools like Taq ...also use polymerases produced using plasmids from Addgene to test for the presence of pathogens in environmental...first round of awards has successfully concluded, Addgene and Seeding Labs will be opening applications for... applicants will propose for plasmids from the Addgene community and hope we can do even more to enable...

  29. How to Deposit Your Plasmids with Addgene

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    ... DNA library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...Reference the Addgene ID in the "Backbone Manufacturer" box for backbones requested through Addgene. For example...for You: Find Articles Citing Addgene Plasmids Resources on the Addgene Website Learn the Benefits of...deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363...strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like to distribute...At Addgene, it is our mission to make it easy for you to share plasmids. To achieve this goal, we will...online, you will need to create an account with Addgene before starting the process. After you have logged...

  30. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

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    Blog Post
    ... a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample 6 grow with...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website Check Out...installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his...developing? 38:00 - End : Dr. Koeris' role on the Addgene Advisory Board and how he hopes to help young biotech...Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check the Other Posts in Our Podcast Series...of our Board of Directors Become a Part of the Addgene Community ...

  31. Why and how to start studying chromatin (with Addgene's help)

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    ...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...in the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on...Chromatin-related genes with plasmids available from Addgene The links below pull up lists of plasmids containing...you can subscribe to these pages to learn when Addgene receives new plasmids containing these genes. As...

  32. Addgene is Expanding Our Viral Vector Service!

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    Blog Post
    ...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

  33. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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    Blog Post
    ...that, when you cite a plasmid from Addgene using its unique Addgene ID number, your publication will be...Feature Browse More Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections...plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to find the plasmids...sophisticated search, and curating plasmid collections. Addgene's "How to cite this plasmid" section Anytime you...repository, we request that you cite it using both its Addgene ID number as well as the reference for its original... process, we explain how to cite plasmids from Addgene right on our plasmid pages and provide a citation...Why do this? Not only does citing plasmids from Addgene help others find them later if they need to use...

  34. QC Sequencing Technologies at Addgene

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    ...Resources on the Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind...technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene keeps up with...Validation: seqWell Addgene’s Tips for Plasmid Quality Control Additional Resources on addgene.org Molecular ...provided a general overview of the QC process at Addgene. All plasmids go through this same initial QC process...Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited plasmids. ...bioinformatic analysis are occurring every year. At Addgene, we want to maintain the highest quality and accuracy... technologies. Sequencing technologies used at Addgene Sanger sequencing (“First-generation sequencing...

  35. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently...plasmids are amplified and none are lost. Each Addgene library has its own depositor transformation and...components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions...a gRNA library, this is less of a concern, but Addgene still recommends plating the transformation mixture...

  36. Celebrate National Book Month with Addgene

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    Blog Post
    ...Communications Manager Addgene's book club Indeed, reading is ingrained into Addgene’s culture. We have a ...still going on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product... of Addgene’s culture. Have you read any of these books? Whether you have or not, let the Addgene community...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...From Addgene Book Club to our on-site book swap/library, reading is part of the culture here at Addgene...and explains the origins of ABC: I founded the Addgene Book Club (aka ABC) in 2011 after having numerous... activities at Addgene ...

  37. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    ...two-color pair. We'll be sending it to Addgene in a matter of days. Addgene: Can you tell us a little more about..., or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many of...available. Visit Ed Boyden lab's Addgene page and click the link in the Addgene Alerts box. References: ...activity of two populations of neurons at once. Addgene spoke to Boyden about this latest optogenetics ...missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can you give...Understanding the brain through optogenetics research Addgene: What are some of the big problems that optogenetic...these tools, so we can focus on the next round. Addgene: What tools are still missing from the optogenetics...

  38. New Viral Vectors - Summer 2024

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    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser...tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to see viral vector reports from other... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

  39. Podcast: A Malawian Professor's Path to Biotech Research

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Seeding Labs Resources on the Addgene Blog Listen to Additional Addgene Podcasts Learn How You Can Contribute...Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research Resources...Resources on Addgene.org Become a part of the Addgene Community ... recently received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that...

  40. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     References...plasmids from this publication at Addgene. Resources on the Addgene Blog Read our Recent Blog Post on...Experiments  Resources on the Addgene Website Find Cepko Lab Plasmids at Addgene ...We recently sat down with Addgene Board Member, depositor, and Harvard Medical School researcher Connie...PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific...

  41. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...Resources on Addgene.org Check out Addgene's synthetic biology page Search for a plasmid Find Addgene's most ...coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large...large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes...to identify synthetic and natural sequences in Addgene’s database. They showed a growing trend of sourcing...In 2006, less than 1% of the genes deposited at Addgene were synthetic. But nine years later, over 20% ...that the most common expression system within the Addgene repository is mammalian, but the largest source...This isn’t the only time researchers have used Addgene as a large data set. Alec A. K. Nielsen & Christopher...

  42. Meet the New Addgene Board Members!

    Type
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    ...This year, Addgene welcomes three new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier...assay technologies makes her a perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics... the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several years ...through this connection that she learned more about Addgene. “The mission to share critical reagents such as...Scientific Officer at KSQ Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically...the lab eventually deposited these plasmids at Addgene. While deposits from for profit organizations are...finds the value in the wealth of resources on Addgene’s website and blog important. “The experience from...

  43. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...Developing the Addgene microbiology community #ASMMicrobe2016 was a great opportunity for Addgene to let microbiologists...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our ...The start of the conference After setting up the Addgene booth on Thursday evening, I stayed to see the ...with lots of researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors...dedicated to science communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a ... about amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...creating well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening...

  44. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

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    Blog Post
    ...cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene. Howarth... and its potential uses. Developing a new tool Addgene: Where did you get the idea to develop SpyLigase...just join one peptide tag to another peptide tag. Addgene: What is the advantage of peptide tags for building...bonds, so there were no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein ...pili that they use to anchor on to host cells. Addgene: Were there any major hurdles along the way? Howarth...capture system.) Cancer and other applications Addgene: What does this technology mean in the context ...capture or for capture using microfluidic devices. Addgene: What other uses do you foresee for this tool? ...

  45. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

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    Blog Post
    ...GIFs, feel free to let us know on Twitter @addgene! Addgene is always looking for new ways to help you...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...coworkers, labmates, and friends as well!   Addgene is sharing so much more than plasmids these days...GIPHY   You can see for yourself at giphy.com/addgene. But that’s not all! You’ll be able to use these...Twitter, Instagram, TikTok and more. Just type “addgene” into the GIF search bar to find that perfect response....   This could be you after watching Addgene’s protocol videos. via GIPHY   If you’re also...

  46. An Inside Look into Addgene's Dev Team

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    Blog Post
    ...all Addgene news Resources on Addgene.org Check out our job postings Learn more about Addgene's mission...about Addgene’s software development team and how we work.  Additional resources on the Addgene blog ...collaborations between Addgene’s Product Team and the stakeholders (other Addgenies). Once we begin work...When you think of Addgene, chances are software development is not one of the things that comes to mind... is a lot of software development happening at Addgene under the hood. A significant portion of our development... benefits. We support everyone that works with Addgene in some way. For users, we just released a major...viral orders are being fulfilled more quickly. As Addgene grows and evolves, we will be here to increase ...

  47. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...of tube for each plasmid we have at Addgene. Each sample at Addgene has four layers of location which can...pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the... a microcentrifuge tube. Once a sample enters Addgene’s lab, a series of events is triggered. Each step...fluctuates throughout the year (see graph below). Addgene scientists inspect each sample and make sure they...takes over and prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities...location There are many different types of samples at Addgene - DNA sent from depositors, bacterial glycerol ...

  48. Transferable Skills Guide: Cross-team Communication

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    Blog Post
    ...communication blog posts Resources on Addgene.org Find careers at Addgene ... project I’d seen in my already long tenure at Addgene. I would work with our research team and software...pinpointed and (mostly) solved two main problems with Addgene’s cross team communication. Although these real ...these plates now” to a fellow team member. But, at Addgene, we use seven different types of “plates” (Fig....not the container was barcoded (this is pretty Addgene specific) The types or purposes of each given container...that there were a lot of words that nearly all Addgene teams used that seemed simple, but they could in...solution for this problem was the creation of an Addgene lexicon. The base lexicon was and continues to ...

  49. Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

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    Blog Post
    ...Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial...Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis...Additional Resources from the Addgene Blog Learn about the Synthetic Biology Education Nonprofit BioBuilder... addgene podcast... Interview with Michael Koeris Resources at Addgene.org Find Plasmids from the Ellis Lab Find Synthetic...

  50. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post
    ...learning.” Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since...work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt, Addgene’s Associate...resources on the Addgene blog Browse our science career blog posts Download Addgene's science career guide...stay in science.” Addgenies Beth Kenkel and Shannon Rinaldi in the Addgene lab! Read their Instagram... guide Resources on Addgene.org Learn more about working at Addgene ...Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member...go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community...
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