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Learn about the latest statistics on CRISPR plasmid deposits and distributions and how this reflects the broader CRISPR community.

Find the basics about chemogenetics: what are chemogenetics, why use them, and how to build your chemogenetic system using receptors and ligands.

Learn how Cpf1 stacks up against Cas9 and NgAgo

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Learn about using SpyLigase for irreversible peptide-peptide ligation and magnetic cancer cell capture.

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Magnetoresponsive proteins have seemed like a dream... but Andrew York's lab has developed and deposited one, opening the possibility of magnetoresponsive tools!

PRIDICT 2.0 is an updated software that can help you design gRNAs for PRIME editing. Read more in our guest post!

Browse Ginkgo Bioworks plasmids to express SARS-CoV-2 viral proteins in several expression systems.

Use this protocol to generate lentivirus

Learn about what's coming for Addgene in 2026

The Patriarchi lab has deposited two new norepinephrine indicators with increased speed and sensitivity.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

If you need to image more than one protein in your sample, multiplex immunofluorescence is an important skill to master!

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The collection can be used for screening transcription factors for cellular programming or cancer drug resistance.

An open-source biosensor platform, TRUPATH, measures the association of G protein signal transducers using bioluminescence resonance energy transfer.

Overview of the CRISPRi system for rewiring metabolic circuitry, with a focus on the paper by Zalatan et al 2015.