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Showing: 81 - 100 of 439 results

  1. Lentivirus Production

    Type
    Protocol
    ...Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding ...

  2. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti...split vector approaches developed in the early 2000’s which allow researchers to deliver oversized cargo...and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression in the K562 cell line (Bak et al....2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...., Alicea, B., Audisio, T. L., Auer, S., Bhardwaj, V., Burgess, S. J., Ferling, I., Gazda, M. A., Hoeppner...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E....Prepare and label a sample that expresses your target(s) at a high level, either naturally or by overexpression... D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant...10.17504/protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction...

  5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...-7_20  Karmakar, S., Panda, D., Panda, S., Dash, M., Saha, R., Das, P., Avinash, S., Shih, J., Yang, ...  References and Resources References Karmakar, S., Panda, D., Baig, M. J., & Molla, K. (2025). A unified...10.1038/s41587-019-0337-2  Molla, K. A., Sretenovic, S., Bansal, K. C., & Qi, Y. (2021). Precise plant genome...s41477-021-00991-1  Qiao, J., Zang, Y., Gao, Q., Liu, S., Zhang, X., Hu, W., Wang, Y., Han, C., Li, D., & ...

  6. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...Nogales presented the first detailed picture of Cas9's three-dimensional shape via x-ray crystallography,...Institute and MIT published another report showing Cas9's structural details. "Understanding this structure ...This Protein Could Change Biotech Forever."  David S. Weiss of Emory University summed it up well in that...

  7. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren.... References and Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A.....67.8.3440-3444.2001 Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R....2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications...

  8. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Western Ontario. Adam summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional... the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous...potential of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...him on Twitter @AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to test if the exogenous...technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 ...Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature...2013 Jan; 493, 429–432. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

  10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019) Gene activation by dCas9-CBP and...

  11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...and resources References Dong D, Guo M, Wang S, Zhu Y, Wang S, Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural...Inhibit DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017),...deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. Phage therapy Phage...doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/...

  12. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ..., C., Baier, A. S., Pascolutti, R., Wegrecki, M., Zheng, S., Ong, J. X., Erlandson, S. C., Hilger, D.,...., Rasmussen, S. G. F., Ring, A. M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display platform...https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu, D. R. (2015). Methods for the directed evolution...nrg3927 Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021). Directed Evolution: Methodologies...://doi.org/10.1021/acs.chemrev.1c00260 Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR...

  13. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin...-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY ... Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL,... . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS...JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and...

  14. Students Invented a New Diagnostic for Lyme Disease — and a Tool for CRISPR Researchers

    Type
    Blog Post
    ...Hsu, E., Gu, W., Miller, S., Pan, C.-Y., Guevara, H., Wadford, D. A., Chen, J. S., & Chiu, C. Y. (2020)...., Ducrotoy, J., Octave, S., Avalle, B., Maffucci, I., & Padiolleau-Lefèvre, S. (2025). Selection and characterization...https://doi.org/10.1038/s41587-020-0513-4  Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X...SHERLOCK and DETECTR CRISPR 101: Cas9 vs. The Other Cas(s) Resources on Addgene.org How to Design a Primer...

  15. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...10.1038/nbt.3947 Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998)....gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are ..., R., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

  16. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...R, Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan G, Whitlow M (1988) Single-chain.../10.1126/science.3140379  Bradbury ARM, Sidhu S, Dübel S, McCafferty J (2011) Beyond natural antibodies...molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific antigen...

  17. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene... full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression...containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors in late March. The ...Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular...

  18. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...Terry, F. E., Roberts, B. J., Rosenberg, A. S., & De Groot, A. S. (2022). In silico Immunogenicity Assessment...www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché, M.-S., Mounho-Zamora, B., LeSauteur, L., & Burns-Naas, ...Manufacturing to Preclinical Studies. In J. Bluemel, S. Korte, E. Schenck, & G. F. Weinbauer (Eds.), The ...

  20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...biotin acceptor site BirA, a biotin ligase a gRNA(s) targeting a single genomic location of interest  ... Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells Table 1: ...methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate of detecting chromatin interactions...they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab...Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In ...
Showing: 81 - 100 of 439 results