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We narrowed to 438 results for: %s

Showing: 81 - 100 of 438 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to test if the exogenous...technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 ...Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature...2013 Jan; 493, 429–432. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...

  2. Lentivirus Production

    Type
    Protocol
    ...Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding ...

  3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan...567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik M (2019) Gene activation by dCas9-CBP and...

  4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...and resources References Dong D, Guo M, Wang S, Zhu Y, Wang S, Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural...Inhibit DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017),...deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. Phage therapy Phage...doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/...

  5. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ..., C., Baier, A. S., Pascolutti, R., Wegrecki, M., Zheng, S., Ong, J. X., Erlandson, S. C., Hilger, D.,...., Rasmussen, S. G. F., Ring, A. M., Manglik, A., & Kruse, A. C. (2018). Yeast surface display platform...https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu, D. R. (2015). Methods for the directed evolution...nrg3927 Wang, Y., Xue, P., Cao, M., Yu, T., Lane, S. T., & Zhao, H. (2021). Directed Evolution: Methodologies...://doi.org/10.1021/acs.chemrev.1c00260 Wilson, D. S., & Keefe, A. D. (2000). Random mutagenesis by PCR...

  6. Mammalian RNAi Tools

    Type
    Collection
    ... Marmari, V., Ebrahimi, M., Rashno, M. M., Abadi, S. J., & Gharagouzlo, E. (2017). Molecular mechanisms... opens in a new window) . Rao, D. D., Vorhies, J. S., Senzer, N., & Nemunaitis, J. (2009). siRNA vs. shRNA...

  7. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...10.1038/nbt.3947 Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998)....gkab1256 Lee, Y., Kim, M., Han, J., Yeom, K., Lee, S., Baek, S. H., & Kim, V. N. (2004). MicroRNA genes are ..., R., Fort, A., Gebhard, C., Goldowitz, D., Guhl, S., Ha, T. J., . . . De Hoon, M. J. L. (2017). An integrated...

  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...R, Hardman K, Jacobson J, Johnson S, Kaufman B, Lee S, Lee T, Pope S, Riordan G, Whitlow M (1988) Single-chain.../10.1126/science.3140379  Bradbury ARM, Sidhu S, Dübel S, McCafferty J (2011) Beyond natural antibodies...molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific antigen...

  9. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin...-Gonzalez A, Zorn K, Gopez A, Hsu E, Gu W, Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY ... Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis BL,... . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS...JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and...

  10. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...Terry, F. E., Roberts, B. J., Rosenberg, A. S., & De Groot, A. S. (2022). In silico Immunogenicity Assessment...www.frontiersin.org/articles/10.3389/fimmu.2022.885672Piché, M.-S., Mounho-Zamora, B., LeSauteur, L., & Burns-Naas, ...Manufacturing to Preclinical Studies. In J. Bluemel, S. Korte, E. Schenck, & G. F. Weinbauer (Eds.), The ...

  11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type
    Blog Post
    ...all SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene... full length and truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression...containing SARS-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors in late March. The ...Plasmids and Resources Page! References Fukushi S, Watanabe R, Taguchi F (2008) Pseudotyped Vesicular...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...biotin acceptor site BirA, a biotin ligase a gRNA(s) targeting a single genomic location of interest  ... Number of loci targeted One Many Sample type(s) Cell lines Cell lines, primary cells Table 1: ...methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased rate of detecting chromatin interactions...they are a large cluster of enhancers. CAPTURE 2.0’s multiplexing and high resolution allowed the Xu Lab...Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In ...

  14. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...Sierra-Mercado, D., Robinson, J. O., Outram, S., Koenig, B. A., Pereira, S., McGuire, A. L., & Lázaro-Muñoz, G....and resources References Dayton, R. D., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer...doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib... Leib, R. D., Cirolia, G., Thomas, D., Stamnes, S., Holt, K., Sinn, P., May, A. P., & Paulk, N. K. (2020...

  15. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...sequence* T2A: (GSG) E G R G S L L T C G D V E E N P G P P2A: (GSG) A T N F S L L K Q A G D V E E N P G...Y A L L K L A G D V E S N P G P F2A: (GSG) V K Q T L N F D L L K L A G D V E S N P G P * (GSG) residues...cloning sites flanking the multicistronic element(s), or you could replace one of the reporter genes above...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...fixed tissue. References 1. Aschauer DF, Kreuz S, Rumpel S. Analysis of transduction efficiency, tropism...PubMed Central PMCID: PMC3785459. 2. Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, Maratos-Flier...21364278. PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

  17. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ... Xiuyuan Ou, et al. that characterized the spike (S) protein of SARS-CoV-2 (Ou et al., 2020). The authors...development of a new plasmid, pCMV14-3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and...References Chu H, Chan JF-W, Yuen TT-T, Shuai H, Yuan S, Wang Y, Hu B, Yip CC-Y, Tsang JO-L, Huang X, Chai...Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of K18-hACE2 Mice Infected...

  18. AAV for Neuronal Tracing

    Type
    Collection
    ... Neuronal Tracing References Wickersham IR, Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal...Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...4.0 license.   An, M., Raguram, A., Du, S. W., Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z....https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. D., Krasnow, N. A., McElroy, A., Tao, Y....Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient site-specific...Coffey, A., Howard, M., Kamath, N., … & Weissman, J. S. (2024). Brainwide silencing of prion protein by AAV-mediated...., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021). Genome-wide programmable transcriptional ...

  20. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...PMCID: PMC2862519. 7. A. L. Firth, T. Menon, G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz...Cooney, N. Malani, T. Brady, J. D. Sander, J. Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman...for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp. E2279–87, Jun. 2013. PubMed...conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp. 14492–6, Sep. 2008. PubMed...PMID: 18787116. PubMed Central PMCID: PMC2567143. 6. S. Radecke, F. Radecke, T. Cathomen, and K. Schwarz,...
Showing: 81 - 100 of 438 results