We narrowed to 161 results for: ALC

1. Mapping the 4D nucleome with CRISPR/Cas9

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   Blog Post

   ...physical map distances of 75 and 2 Mbp, with the calculated fluorescent distances correlating with the previously...

2. 28 Hot Plasmid Technologies from 2015

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   ...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...

3. Hot Plasmids and Viral Preps - July 2021

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   ...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

4. Sensing Neuronal Dopamine

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   Blog Post

   ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

5. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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   Blog Post

   .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between...

8. Multifunctional Neural Fibers: Modulation from Gut to Brain

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   Blog Post

   ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

9. Plasmid Cloning by PCR

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   Blog Post

   ...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

11. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    ...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...

12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post

    ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

13. FPbase: A new community-editable fluorescent protein database

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    Blog Post

    ...feature filter spectra and collection efficiency calculations.  With ~80 FP spectra (and rapidly growing, ...

14. Visualizing Translation at the Single Molecule Level

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    ...inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5 codons/second...

15. Addgene Depositors Get More Citations

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    Blog Post

    ... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...

16. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post

    ...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...

17. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...

18. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...

19. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ...

20. CRISPRainbow and Genome Visualization

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    Blog Post

    ...physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously...