Addgene

We narrowed to 161 results for: ALC

Showing: 81 - 100 of 161 results

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Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

Type

Blog Post

.... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

Type

Blog Post

...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Type

Blog Post

...genomes, such as the malaria-causing Plasmodium falciparum. Throughout these talks, I oscillated between...
28 Hot Plasmid Technologies from 2015

Type

Blog Post

...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming soon!). Takai, et al...
Multifunctional Neural Fibers: Modulation from Gut to Brain

Type

Blog Post

... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...
Plasmid Cloning by PCR

Type

Blog Post

...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Type

Blog Post

...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Type

Blog Post

... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...
FPbase: A new community-editable fluorescent protein database

Type

Blog Post

...feature filter spectra and collection efficiency calculations. With ~80 FP spectra (and rapidly growing, ...
Plasmids 101: NGS Quality Control for Pooled Libraries

Type

Blog Post

...wealth distribution” curve, or Lorenz curve. By calculating the area under the curve (AUC), one can gain ...
Visualizing Translation at the Single Molecule Level

Type

Blog Post

...inhibitor that stalls ribosomes after initiation, to calculate a ribosome translocation rate of ~3.5 codons/second...
Addgene Depositors Get More Citations

Type

Blog Post

... of publication. Table 1 provides an example calculation of the weights. Table 1: Journal Weighting Example...
Predicting Adverse Reactions to Monoclonal Antibody Drugs

Type

Blog Post

...algorithms are trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al...
Plasmids for Stem Cell Research

Type

Collection

...fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Mol...
A Practical Approach to Choosing the B(right)est Fluorescent Protein

Type

Blog Post

...) respectively. The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

...wild type target in each sample can be quickly calculated (details in [2]) and can be used to quantify ...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...
CRISPRainbow and Genome Visualization

Type

Blog Post

...physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances correlating with the previously...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...INTRSECT project. Check out the new additions. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...
Viral Vectors 101: Chemogenetics

Type

Blog Post

...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...

Showing: 81 - 100 of 161 results

Results per page:

Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

28 Hot Plasmid Technologies from 2015

Multifunctional Neural Fibers: Modulation from Gut to Brain

Plasmid Cloning by PCR

Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

FPbase: A new community-editable fluorescent protein database

Plasmids 101: NGS Quality Control for Pooled Libraries

Visualizing Translation at the Single Molecule Level

Addgene Depositors Get More Citations

Predicting Adverse Reactions to Monoclonal Antibody Drugs

Plasmids for Stem Cell Research

A Practical Approach to Choosing the B(right)est Fluorescent Protein

Screening for Successful Genome Editing with Digital PCR

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

CRISPRainbow and Genome Visualization

Hot Plasmids and Viral Preps - March 2021

Viral Vectors 101: Chemogenetics

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