We narrowed to 95 results for: CAG

1. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

   Type

   Blog Post

   ... damage results in the transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells...

2. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve ...

3. AAV ddPCR Titration

   Type

   Protocol

   ...probe targeting ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT...

4. 28 Hot Plasmid Technologies from 2015

   Type

   Blog Post

   ...phototropin 1 (AsLOV2). In the dark, this peptide is caged by the asLOV2 domain and has reduced affinity for...

5. Zinc Finger Consortium: Zinc Finger Arrays

   Type

   Collection

   ...OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1 OZ515 and OZ516 OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517...OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond... CODA tGTCTTCATCTCCTTCAGATGCTGTCc fam82a2 OZ573 and OZ574 CODA CODA gCACCGCTGCAGTAAGAGCTGAGGAAt morc3b...CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc zgc:162148...OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt yap1 OZ589 and OZ590 CODA OPEN gCCCAGCTGCAGGGAGGCAGGTGAGg pdeb3 OZ591...OPEN CODA gGTCCGCCTCAGGTAGAGGGTGAGg col6a2 OZ593 and OZ594 CODA CODA aGTCCTCCGATGGCCCAGGTGATGTTc SDC3 OZ595...CODA CODA tGGCAGCAGCTTTTCGAAGAATAGg zgc:65890 OZ599 and OZ600 OPEN CODA aATCTTCCACAGTGATGAGGACGTGc panx2 ...

6. Synthetic Biology - Assembly Standards Guide

   Type

   Collection

   ... EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: TACTAGAG...Enzymes: EcoR1 , NotI , Spel Suffix: GCTAGC GCGGCCG CTGCAG Suffix Enzymes: Nhel , NotI , PstI Scar: GCTAGT... EcoR1 , NotI , XbaI Suffix: T ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: SpeI , NotI , PstI Scar: ACTAGA..., (NgoMIV) Suffix: ACCGGT TAAT ACTAGT A GCGGCCG CTGCAG Suffix Enzymes: Agel , Spel , Notl, Pstl Scar: ...

7. Retrovirus Plasmids

   Type

   Collection

   ...-expressing envelope plasmid. Didier Trono 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid. Arthur... Arthur Nienhuis and Patrick Salmon 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis...

8. Genetic Code Expansion

   Type

   Collection

   ...TAG Huiwang Ai 71404 pMAH2-CageCys leucyl-tRNA-synthetase E. coli photocaged cysteine Mammalian TAG Huiwang...140022 pAS_4xMma PylT_FLAG-PcKRS PcKRS M. barkeri photocaged-lysine Mammalian TAG Simon Elsaesser 140023 pAS...

9. Arf GTPase Family

   Type

   Collection

   ... Mammalian (pcDNA4c, pCAG), Gateway GEF Arfgef2 (BIG2) 10564 1785 Mammalian (pCAG), Gateway GEF Arfgef3...

10. Lentivirus Plasmids

    Type

    Collection

    ...packaging construct encoding tat . Jakob Reiser 35617 pCAG-Eco Ecotropic MLV expressing envelope plasmid. Arthur...packaging construct encoding tat . Jakob Reiser 35616 pCAG-VSVG VSV-G-expressing envelope plasmid. Arthur Nienhuis...

11. Fluorescent Protein Guide: Subcellular Localization

    Type

    Collection

    ...Membranes Palmitoylation EGFP Connie Cepko 14757 pCAG-mGFP Membranes Palmitoylation sequence from GAP43...Actin filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin filaments beta-Actin EGFP Ryohei...

12. CRISPR Plasmids - Empty gRNA Vectors

    Type

    Collection

    ...Mb Cpf1 Kim pCAG-SpCas9-GFP-U6-gRNA 79144 Mammalian hU6 yes, cut S. pyogenes EGFP Zou pCAG-eCas9-GFP-U6...

13. Sequencing Primers

    Type

    Guide

    ...M13 Forward (-21) TGTAAAACGACGGCCAGT In lacZ gene Forward M13 Reverse CAGGAAACAGCTATGAC In lacZ gene Reverse... Forward CCCAGTCACGACGTTGTAAAACG In lacZ gene Foward M13/pUC Reverse AGCGGATAACAATTTCACACAGG In lacZ gene...MSCV Reverse CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus Reverse pBABE 5' CTTTATCCAGCCCTCAC Psi packaging... AC5 ACACAAAGCCGCTCCATCAG Drosophila Actin 5C promoer Forward Alpha-factor TACTATTGCCAGCATTGCTGC Alpha...Reverse LacZ-R GACAGTATCGGCCTCAGGAA 5' end of LacZ Reverse LexA CGTCAGCAGAGCTTCACCATTG 3' end of LexA ...LucNrev CCTTATGCAGTTGCTCTCC 5' end of luciferase Reverse M13 Forward (-21) TGTAAAACGACGGCCAGT In lacZ ...M13 Forward (-40) GTTTTCCCAGTCACGAC In lacZ gene Forward M13 Reverse CAGGAAACAGCTATGAC In lacZ gene Reverse...

14. Immunology Research Plasmids and Resources

    Type

    Collection

    ...receptor 3 - GAST gastrin GAS GCG glucagon GLP1, GLP2, GRPP GCGR glucagon receptor GGR, MGC138246 GDF1 growth...MGC70354, foveolin GLP1R glucagon-like peptide 1 receptor MGC138331 GLP2R glucagon-like peptide 2 receptor...

15. Optogenetics Guide

    Type

    Guide

    ...Light-inducible Dronpa mutant domains that associate and cage a protein in the dark, while dissociate and activate...